(A–C) H and E staining of horizontal sections of E18.5 larynges. (A’–C’) Diagrams of anatomy shown in (A–C). Fuz mutant larynges (B–B’) are significantly altered compared to controls, (A–A’). Connective tissue in mutants (light green, (C’) is increased in mutants compared to controls (A–A’), while cartilage and muscle are irregularly patterned. Gli3-/- larynges are less altered than Fuz-/- embryos compared to controls (A–A’). Thyroglottal Connective tissue appears increased in Gli3 mutants (light green, red arrow, (C’). Scale bars indicate 500 μm. Abbreviations: (AC) Arytenoid Cartilage, (CC) Cricoid Cartilage, (CT) Cricothyroid muscle, (G) Glottis, (L) Larynx, (LCA) Lateral Cricoarytenoid muscle, (PCA) Posterior Cricoarytenoid muscle, (T) Tongue, (TAM) Thyroarytenoid Muscle, (TC) Thryoid Cartilage, (TgCT) Thyroglottal connective tissue, (Tr) Trachea, (VL) Vocal Ligament, (VM) Vocalis Muscle, (VF) Vocal fold. (V) and (D) indicate dorso-ventral axes.