Magnetogenetics: Problems on the back of an envelope
- Massachusetts Institute of Technology, United States
Many biophysicists have in their library a little book by Howard Berg called Random Walks in Biology. In this book Berg applies principles one learns in an introductory physical chemistry class to analyze processes ranging from the migration of proteins on gels to the motility of whole living cells (Berg, 1993). In addition to exploring a specific set of phenomena, the book promotes the ethos of using simple models and calculations – often so simple that they could be performed on "the back of the envelope" – to bring order to otherwise messy biological systems. This ethos gives life scientists a quantitative alternative to the qualitative empiricism that is found in many fields of modern biology.
Now, in eLife, Markus Meister of Caltech reports how the results of such calculations have led him to question a number of recent papers about the use of magnetic fields to manipulate proteins, cells and organisms (Meister, 2016). Because biological tissue normally interacts only weakly with magnetic fields, it might be possible to use magnetically-active molecules that interface with biological systems to remotely control specific physiological processes. Given the wealth of research made possible by optogenetics – in which light is used to control cells that have been genetically modified to make them sensitive to light (Adamantidis et al., 2015) – one can only imagine the possibilities afforded by an analogous “magnetogenetic” approach. The papers that Meister critiques all claim to harness iron-binding proteins to realize various aspects of magnetogenetics. However, using arguments from elementary physics, he shows that key results reported in these articles cannot be explained by the magnetic phenomena the authors say they have exploited (Figure 1).
Figure 1
Molecular-scale magnetic interaction energies.
The energies associated with various magnetogenetic components, as estimated optimistically by Meister, are labeled in magenta. Each is orders of magnitude smaller than the thermal energy per degree of freedom at room temperature (dark blue). In contrast, based on our calculations, interactions involving magnetite particles (Fe3O4; light blue) can exceed the thermal energy (kT) at room temperature. These calculations assume that the magnetization of magnetite is 480 emu/cm3 and that the specific loss power for an individual particle is 24 fW. Note that the x-axis is logarithmic. The strength of the earth's magnetic field varies between about 25 and 65 microtesla at the earth's surface. mT: millitesla; B: magnetic field; k: Boltzmann’s constant; T: temperature.
In the first paper, Can Xie of Peking University and co-workers report that Isca1 – an iron-sulfur cluster protein that is found in Drosophila – acts like a compass needle and aligns with the Earth’s magnetic field (Qin et al., 2015). Such a "biocompass" could be a potential molecular building block for magnetogenetics. However, Meister’s calculations show that Isca1 (Cózar-Castellano et al., 2004) is not capable of acting like a compass needle. The iron content of Isca1 was proposed as a basis for magnetic effects. Certain configurations of iron give rise to molecular-scale magnetic dipoles, which interact with magnetic fields in roughly the same way a miniscule bar magnet would. But Meister points out that the smallest iron-containing species with dipoles strong enough to spontaneously orient in magnetic fields at room temperature are mineral particles with diameters of ~20 nm, each of which contains hundreds of thousands of iron atoms in very close proximity to each other. Both the quantity and closeness of the iron atoms in such nanoparticles are necessary in order for the magnetic contributions of the individual atoms to cooperate with each other sufficiently to override the randomizing effects of thermal energy (Cullity and Graham, 2008), and the 40 iron atoms in Isca1 are too few and too far apart to achieve this. Even if the atoms were close enough, Meister calculates that the interaction between the protein and the earth's magnetic field would be much too weak (by a factor of about 105) to overcome thermal fluctuations at room temperature.
Rather than searching for naturally occurring building blocks for magnetogenetics, other researchers have used genetic techniques to produce hybrid protein assemblies that contain the iron storage protein ferritin conjugated to an ion channel. Ali Güler of the University of Virginia and co-workers (Wheeler et al., 2016) studied neurons in which the ferritin was conjugated to an ion channel called TRPV4 that is activated by mechanical force (Liedtke, 2008). Güler and co-workers wanted to use magnetic fields to rotate or pull on the ferritin components, thus exerting a force that could be used to trigger the opening of the ion channel. The researchers reported that they observed electrophysiological and behavioral changes when they applied magnetic field gradients to various systems, including in vitro systems, brain slices and freely moving mice. However, Meister’s analysis indicates that the magnetic field conditions used in the study could produce forces that were, at most, nine orders of magnitude lower than the forces needed to open known mechanically-sensitive ion channels. Indeed, the forces are too weak (by several orders of magnitude) to overcome random thermal fluctuations at room temperature, and are thus unlikely to mediate magnetogenetics.
Jonathan Dordick (Rensselaer Polytechnic Institute), Jeffrey Friedman (Rockefeller University) and co-workers (Stanley et al., 2015) studied insulin-secreting cells in which the ferritin was conjugated to a temperature-sensitive ion channel called TRPV1 (Caterina et al., 1997). The basic idea was that applying an oscillating magnetic field would heat the ferritin and that the resulting increase in temperature would open the ion channel, triggering insulin release. The approach is inspired by a similar strategy, called magnetic hyperthermia, that uses oscillating fields to heat much more strongly magnetic particles, and can be used to treat cancer (Pankhurst et al., 2009). Stanley et al. reported that ion channels in mice could be activated by oscillating magnetic fields. However, Meister estimated the specific loss power – the amount of heat dissipated by a particle per gram of iron – for the ferritin nanoparticles (Fantechi et al., 2014) used in the experiments and found that it was too low to support a magnetogenetic approach.
Where does magnetogenetics go from here? Two ways forward seem plausible. The first is to continue efforts to control biological systems using magnetic species that are far more potent than Isca1 or ferritin. Indeed, two groups have already demonstrated that nanoparticles with magnetic moments ~1000 times higher than those of ferritin can be used to manipulate neural activity (Huang et al., 2010; Chen et al., 2015). Using magnetic fields to heat such nanoparticles in close proximity to temperature-sensitive ion channels should lead to a physically practical form of the technique proposed by Stanley et al. Another challenge is to deliver these nanoparticles to where they are needed: in the future, it might be possible to do this by repurposing the genetic machinery that enables certain bacterial and vertebrate species to produce their own magnetite (Johnsen and Lohmann, 2005). A second way forward might involve a critical re-examination of the experiments performed by Wheeler et al. and Stanley et al. If their cellular-level findings prove reproducible, the true mechanism that explains them might even fulfill some of the promise that magnetogenetics seemed to offer before it met the back of Professor Meister’s envelope.
References
-
Optogenetics: 10 years after ChR2 in neurons--views from the communityNature Neuroscience 18:1202–1212.https://doi.org/10.1038/nn.4106
-
Wireless magnetothermal deep brain stimulationScience 347:1477–1480.https://doi.org/10.1126/science.1261821
-
BookIntroduction to Magnetic Materials. 2nd EdnWiley: IEEE Press.https://doi.org/10.1002/9780470386323
-
hIscA: a protein implicated in the biogenesis of iron–sulfur clustersBiochimica Et Biophysica Acta 1700:179–188.https://doi.org/10.1016/j.bbapap.2004.05.004
-
The physics and neurobiology of magnetoreceptionNature Reviews Neuroscience 6:703–712.https://doi.org/10.1038/nrn1745
-
Molecular mechanisms of TRPV4-mediated neural signalingAnnals of the New York Academy of Sciences 1144:42–52.https://doi.org/10.1196/annals.1418.012
-
Progress in applications of magnetic nanoparticles in biomedicineJournal of Physics D: Applied Physics 42:224001.https://doi.org/10.1088/0022-3727/42/22/224001
-
Genetically targeted magnetic control of the nervous systemNature Neuroscience 19:756–761.https://doi.org/10.1038/nn.4265
Article and author information
Author details
Publication history
Copyright
© 2016, Anikeeva et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 4,943
- views
-
- 578
- downloads
-
- 17
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Magnetogenetics: Problems on the back of an envelope
eLife 5:e19569.
https://doi.org/10.7554/eLife.19569
Further reading
-
- Developmental Biology
- Physics of Living Systems
Shape changes of epithelia during animal development, such as convergent extension, are achieved through the concerted mechanical activity of individual cells. While much is known about the corresponding large-scale tissue flow and its genetic drivers, fundamental questions regarding local control of contractile activity on the cellular scale and its embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry to local tension in recently obtained time-lapse imaging data of gastrulating Drosophila embryos. This analysis systematically decomposes cell shape changes and T1 rearrangements into internally driven, active, and externally driven, passive, contributions. Our analysis provides evidence that germ band extension is driven by active T1 processes that self-organize through positive feedback acting on tensions. More generally, our findings suggest that epithelial convergent extension results from the controlled transformation of internal force balance geometry which combines the effects of bottom-up local self-organization with the top-down, embryo-scale regulation by gene expression.
-
- Physics of Living Systems
The coordinated motion of animal groups through fluids is thought to reduce the cost of locomotion to individuals in the group. However, the connection between the spatial patterns observed in collectively moving animals and the energetic benefits at each position within the group remains unclear. To address this knowledge gap, we study the spontaneous emergence of cohesive formations in groups of fish, modeled as flapping foils, all heading in the same direction. We show in pairwise formations and with increasing group size that (1) in side-by-side arrangements, the reciprocal nature of flow coupling results in an equal distribution of energy requirements among all members, with reduction in cost of locomotion for swimmers flapping inphase but an increase in cost for swimmers flapping antiphase, and (2) in inline arrangements, flow coupling is non-reciprocal for all flapping phase, with energetic savings in favor of trailing swimmers, but only up to a finite number of swimmers, beyond which school cohesion and energetic benefits are lost at once. We explain these findings mechanistically and we provide efficient diagnostic tools for identifying locations in the wake of single and multiple swimmers that offer opportunities for hydrodynamic benefits to aspiring followers. Our results imply a connection between the resources generated by flow physics and social traits that influence greedy and cooperative group behavior.
