Evidence for evolutionary divergence of activity-dependent gene expression in developing neurons
- University of Edinburgh, United Kingdom
- Institute for Stem Cell Biology and Regenerative Medicine, National Centre for Biological Sciences, India
- UCL Institute of Neurology, United Kingdom
- The Francis Crick Institute, United Kingdom
- Imperial College, United Kingdom
Figures
Figure 1 with 1 supplement
Conservation and divergence in gene regulation in neurons of human and mouse origin.
(A,B) Analysis of gene expression changes induced by KCl/FPL in Hum-ESCCORT-neurons (A) and DIV10 Mus-PRIMCORT-neurons (B). Normalised RNA-seq read density (FPKM) mapping to each gene in RNA …
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Figure 1—source data 1
Data set relating to Figure 1a–e.
- https://doi.org/10.7554/eLife.20337.004
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Figure 1—source data 2
Data set relating to Figure 1—figure supplement 1a.
- https://doi.org/10.7554/eLife.20337.005
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Figure 1—source data 3
Data set relating to Figure 1—figure supplement 1b.
- https://doi.org/10.7554/eLife.20337.006
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Figure 1—source data 4
Data set relating to Figure 1—figure supplement 1c–d.
- https://doi.org/10.7554/eLife.20337.007
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Figure 1—source data 5
Data set relating to Figure 1—figure supplement 1e–f.
- https://doi.org/10.7554/eLife.20337.008
Figure 1—figure supplement 1
Conservation and divergence in gene regulation in neurons of human and mouse origin.
(A) Example fluo-3 Ca2+ imaging trace of KCL/FPL-treated Hum-ESCCORT-neurons. (B) qPCR analysis of the fold induction of the indicated genes in Hum-ESCCORT-neurons after 4h of KCl/FPL treatment. …
Figure 2 with 1 supplement
Stem cell origin does not substantially impact on activity-dependent gene responsiveness.
(A) Example immunofluorescence pictures of Mus-ESCCORT-neurons stained for neuronal markers Tuj1 (upper) and Reelin (lower). Note also absence of Nestin staining (upper), a marker of …
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Figure 2—source data 1
Data set relating to Figure 2e–g.
- https://doi.org/10.7554/eLife.20337.011
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Figure 2—source data 2
Data set relating to Figure 2—figure supplement 1e.
- https://doi.org/10.7554/eLife.20337.012
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Figure 2—source data 3
Data set relating to Figure 2—figure supplement 1b–d.
- https://doi.org/10.7554/eLife.20337.013
Figure 2—figure supplement 1
Differential gene inducibility is not linked to basal levels of gene expression.
(A) Normalised RNA-seq read density (FPKM) mapping to each gene in RNA extracted from control vs. KCl/FPL-treated Mus-ESCCORT-neurons is shown (n = 3 independent biological replicates). Genes whose …
Figure 3 with 1 supplement
DNA sequence is a contributing factor to species-dependent gene responsiveness to neuronal activity.
(A) Analysis of gene expression changes induced by KCl/FPL in mouse Tc1 neurons. Mouse genes are shown in grey, human chromosome-21 (Hsa-21) genes in red. (B) The graph concerns 72 orthologous pairs …
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Figure 3—source data 1
Data set relating to Figure 3—figure supplement 1a.
- https://doi.org/10.7554/eLife.20337.016
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Figure 3—source data 2
Data set relating to Figure 3b.
- https://doi.org/10.7554/eLife.20337.017
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Figure 3—source data 3
Data set relating to Figure 3c–f.
- https://doi.org/10.7554/eLife.20337.018
Figure 3—figure supplement 1
Inducibility of mouse genes in wild-type and Tc1 neurons strongly correlate.
(A) Correlation of KCl/FPL-induced fold-change in the same 11,302 mouse genes analysed in Figures 1 and 2,in DIV10 Mus-ESCCORT-neurons from wild-type vs. Tc1 primary cortical neurons, showing the …
