Evidence for evolutionary divergence of activity-dependent gene expression in developing neurons

  1. Jing Qiu
  2. Jamie McQueen
  3. Bilada Bilican
  4. Owen Dando
  5. Dario Magnani
  6. Karolina Punovuori
  7. Bhuvaneish T Selvaraj
  8. Matthew Livesey
  9. Ghazal Haghi
  10. Samuel Heron
  11. Karen Burr
  12. Rickie Patani
  13. Rinku Rajan
  14. Olivia Sheppard
  15. Peter C Kind
  16. T Ian Simpson
  17. Victor LJ Tybulewicz
  18. David JA Wyllie
  19. Elizabeth MC Fisher
  20. Sally Lowell
  21. Siddharthan Chandran  Is a corresponding author
  22. Giles E Hardingham  Is a corresponding author
  1. University of Edinburgh, United Kingdom
  2. Institute for Stem Cell Biology and Regenerative Medicine, National Centre for Biological Sciences, India
  3. UCL Institute of Neurology, United Kingdom
  4. The Francis Crick Institute, United Kingdom
  5. Imperial College, United Kingdom
3 figures

Figures

Figure 1 with 1 supplement
Conservation and divergence in gene regulation in neurons of human and mouse origin.

(A,B) Analysis of gene expression changes induced by KCl/FPL in Hum-ESCCORT-neurons (A) and DIV10 Mus-PRIMCORT-neurons (B). Normalised RNA-seq read density (FPKM) mapping to each gene in RNA …

https://doi.org/10.7554/eLife.20337.003
Figure 1—figure supplement 1
Conservation and divergence in gene regulation in neurons of human and mouse origin.

(A) Example fluo-3 Ca2+ imaging trace of KCL/FPL-treated Hum-ESCCORT-neurons. (B) qPCR analysis of the fold induction of the indicated genes in Hum-ESCCORT-neurons after 4h of KCl/FPL treatment. …

https://doi.org/10.7554/eLife.20337.009
Figure 2 with 1 supplement
Stem cell origin does not substantially impact on activity-dependent gene responsiveness.

(A) Example immunofluorescence pictures of Mus-ESCCORT-neurons stained for neuronal markers Tuj1 (upper) and Reelin (lower). Note also absence of Nestin staining (upper), a marker of …

https://doi.org/10.7554/eLife.20337.010
Figure 2—figure supplement 1
Differential gene inducibility is not linked to basal levels of gene expression.

(A) Normalised RNA-seq read density (FPKM) mapping to each gene in RNA extracted from control vs. KCl/FPL-treated Mus-ESCCORT-neurons is shown (n = 3 independent biological replicates). Genes whose …

https://doi.org/10.7554/eLife.20337.014
Figure 3 with 1 supplement
DNA sequence is a contributing factor to species-dependent gene responsiveness to neuronal activity.

(A) Analysis of gene expression changes induced by KCl/FPL in mouse Tc1 neurons. Mouse genes are shown in grey, human chromosome-21 (Hsa-21) genes in red. (B) The graph concerns 72 orthologous pairs …

https://doi.org/10.7554/eLife.20337.015
Figure 3—figure supplement 1
Inducibility of mouse genes in wild-type and Tc1 neurons strongly correlate.

(A) Correlation of KCl/FPL-induced fold-change in the same 11,302 mouse genes analysed in Figures 1 and 2,in DIV10 Mus-ESCCORT-neurons from wild-type vs. Tc1 primary cortical neurons, showing the …

https://doi.org/10.7554/eLife.20337.019

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