Plasmodium falciparum ligand binding to erythrocytes induce alterations in deformability essential for invasion
- The Walter and Eliza Hall Institute of Medical Research, Australia
- Washington University School of Medicine, United States
- Hawaii Pacific University, United States
- The University of Melbourne, Australia
- The Queen's Medical Center and John A. Burns School of Medicine, University of Hawaii, United States
Figures
Figure 1 with 2 supplements
P. falciparum EBA-175 RII binding to GPA increases deformability of the erythrocyte.
(A) Live imaging time frames showing a merozoite deforming an erythrocyte. Scale bar 5 μm. (B) Atomic force microscopy (AFM) screen of the effect of P. falciparum invasion ligands on the erythrocyte …
Figure 1—figure supplement 1
Binding of P. falciparum recombinant ligands to human erythrocytes.
(A) Schematics showing EBA-175, PfRh4 and PfRh5 domain structure. The black bars represent the recombinant fragments spanning the binding domains used in this study. (B) Coomasie gel of the …
Figure 1—figure supplement 2
Binding of recombinant EBA-140 RII to human erythrocytes.
(A) Quantitative binding assay of a 6x-His tagged recombinant EBA-140 RII. Erythrocytes were labeled with recombinant RII-140 and stained with a FITC conjugated anti-6x-His antibody. (B) …
Figure 2 with 2 supplements
EBA-175 RII induces an increase in phosphorylation of trans-membrane and cytoskeletal erythrocyte proteins.
(A–B) MW vs. pI 2D electrophoresis gel autoradiographs of EBA-175 RII treated (B) or untreated (A) 32P radio-labeled erythrocyte ghosts. (C–F) Western blot phosphorylation validation of tropomodulin …
Figure 2—figure supplement 1
Binding of EBA-175 tohuman erythrocytes activates increased phosphorylation of the host cytoskeleton.
(A) Autoradiographs overlay of MW vs. pI 2D electrophoresis gel of EBA-175 treated (red) or untreated (green) 32P radio-labeled RBC ghosts. (B) Coomasie blue stained preparative 2-D gel of untreated …
Figure 2—figure supplement 2
Quantitation and identification of peptides phosphorylated after EBA-175 binding to human erthrocytes.
(A) Stacked bar graph (left) of the total number of unique peptides detected (982) in the H + L mix plotting the percentage of no phosphorylated peptides (0P) and with single (1P), double (2P) and …
Figure 3
FTY720 and Waixenicin A, inhibitors of TRPM7, block P. falciparum erythrocyte invasion.
(A) Parasitemia after 12–16 hr post addition of synchronous schizonts to erythrocytes treated with different inhibitors at 50 μM. Parasitaemia was normalized to the control. Error bars show SEM …
Figure 4
FTY720 inhibits P. falciparum invasion by interfering with a post-translational pathway triggered by EBA-175 binding to GPA.
(A) Phosphopeptide heavy (H) to light (L) ratios of EBA-175 (H) with PBS (L) treated ghosts (left). Phosphopeptide intermediate (M) to light (L) ratios of EBA-175 and FTY720 treated (M) with …
Videos
Video 1
This video is linked to Figure 4F which shows the still time lapse images of this video-microscopy of P. falciparum merozoites invading Bodipy TR Ceramide labelled human erythrocytes.
This shows the deformation caused by the merozoites during the initial interaction before invasion is activated.
Video 2
This video is linked to Figure 4F which shows the still time lapse images of this video-microscopy of P. falciparum merozoites attempting to invade Bodipy TR Ceramide labelled human erythrocytes in the presence of FTY720.
This shows the lack of severe deformation caused by the merozoites during the initial interaction and their inability to invade. This is linked to Figure 4F which shows the still time lapse images …
Additional files
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Supplementary file 1
Table of LC-MSMS evidence and quantitative statistics for the double labelling experiment in which EBA-175 treated erythrocyte proteins were labelled with heavy isotope (H) versus untreated that were labelled with light isotope (L).
- https://doi.org/10.7554/eLife.21083.012
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Supplementary file 2
Table of LC-MSMS evidence and quantitative statistics for the triple labelling experiment in which EBA-175 treated erythrocytes were labelled with heavy isotope (H), EBA-175+FTY720 treated erythrocyte proteins were labelled with medium isotope (M) versus untreated erythrocyte proteins labelled with light isotope (L).
- https://doi.org/10.7554/eLife.21083.013
