(A) RNAi of MIRAGE1, cep-1/p53 or spo-11 partially suppresses sterility of hpl-2, let-418, and lin-13. Average number of progeny per worm for control empty vector RNAi (EV) or the indicated RNAi treatments in hpl-2(tm1489), let-418(n3536), or lin-13(n770) (averages of 5–11 experiments). Experiments were done under conditions where the mutant strain was nearly sterile to detect an increase in fertility (see Materials and methods). Control progeny numbers vary by experiment, but were always paired with experimental RNAi. Stars indicate statistical significance assessed using paired t-tests, comparing experimental to control RNAi (p<0.05, one star; p<0.01, two stars; p<0.001, three stars). Two sets of RNAi clones were used to target MIRAGE1 elements (termed mirage-A and mirage-B). RNAi clones used are given in the methods. (B) Mutation of cep-1/p53 partially suppresses sterility of hpl-2, let-418, and lin-13 mutants at 25°C. Statistical significance was assessed using single sided t-tests, asking if cep-1; hpl-2, cep-1; let-418, and cep-1; lin-13 double mutants had larger broods than the corresponding heterochromatin single mutants. See methods for growth conditions. (C) Loss of cep-1 partially rescues growth delay defect of heterochromatin mutants at 25°C. Developmental stage of worms grown from L1 at 25C for approximately 48 hr was assessed (adult, L4, younger than L4). A representative experiment out of three replicates is shown, assaying between 95 and 213 worms in each. See methods for growth conditions.