Peptidoglycan sensing by octopaminergic neurons modulates Drosophila oviposition
- Aix-Marseille Université, Centre National de la Recherche Scientifique, UMR 7288, Institut de Biologie du Développement de Marseille, France
Figures
Figure 1 with 3 supplements
PGN-mediated NF-κB pathway activation decreases female oviposition.
(A) Schematic representation of the Drosophila IMD pathway. Peptidoglycan (PGN) released by bacteria is recognized at the cell membrane by PGRP-LC or inside the cell by PGRP-LE. This PGRP/PGN …
Figure 1—figure supplement 1
Infection by bacteria decreases female oviposition.
(A) The effect of bacterial infection on egg laying is dose-dependent and reversible. Egg-laying ratio of wt (A) and PGRP-LB mutants (B) quantified after septic injury with various E. coli …
Figure 1—figure supplement 2
IMD pathway overactivation leads to an exacerbated oviposition drop following exposure to bacteria.
(A) The exacerbated oviposition drop following bacterial infection in PGRP-LB mutant is also observed in transheterozygote PGRP-LB mutants. (B) The exacerbated phenotype observed in PGRP-LB mutant …
Figure 1—figure supplement 3
Bacterial infection provokes an accumulation of mature oocytes in female ovaries.
(A) Diagram representing ovaries containing ovarioles and oocytes within a single ovariole. (B) Following septic injury, stage 14 oocytes accumulate in ovaries. Pictures of opened ovaries with …
Figure 2 with 1 supplement
One specific PGRP-LB isoform controls oviposition.
(A) PGRP-LB locus organization. Three PGRP-LB isoforms are produced from the locus. The green lines represent the cloned fragments used to generate the Gal4 constructs. (B) Egg-laying ratio of PGRP-L…
Figure 2—figure supplement 1
While all PGRP-LB isoforms possess amidase activity, PGRP-LBPD isoform is not required for the negative regulation of the systemic immune response.
(A) Quantification of Diptericin mRNA in wt and PGRP-LB mutant guts expressing the different PGRP-LB isoforms under the control of the gut specific Gal4 driver, NP1Gal4 and orally infected with Ecc15…
Figure 3
A functional NF-κB pathway is required in pLB1 cells to modulate egg-laying post-infection.
(A) Egg-laying ratio of wt females in which the IMD pathway has been inactivated in pLB1 cells only (pLB1Gal4/UAS-RelIR and pLB1Gal4/UAS-PGRP-LEIR)(B) Egg-laying ratio of PGRP-LB mutant females in …
Figure 4
pLB1 is expressed in cells of the nervous system.
(A) Diagram summarizing pLB1 expression pattern (green lines) in the brain (A’), the Ventral Nerve Cord (VNC) (A’’) and the ovaries (A’’’). Expression pattern of pLB1QF/QUAS-GFP (pLB1>GFP) in …
Figure 5 with 1 supplement
Some pLB1 cells are neurons controlling oviposition via NF-κB.
(A) Egg-laying ratio of PGRP-LB mutant females in which the pan-neuronal Gal4 drivers (Elav Gal4) is used to drive expression of the PGRP-LBPD isoform. Expression of the PGRP-LBPD isoform in neurons …
Figure 5—figure supplement 1
ppk, Ilp7, prospero or voila-positive cells do no regulate female egg laying after infection.
(A) Egg-laying ratio of wt mated females in which the IMD pathway has been specifically inactivated via UAS-FaddIR ectopic expression in ppk, Ilp7, prospero or voila-positive cells. (B) While …
Figure 6 with 1 supplement
Bacteria modulate egg-laying behavior via the octopamine pathway.
(A) Septic injury reduces egg laying in PGRP-LB virgin females. Egg-laying ratio post-septic injury of wt and PGRP-LB mutant virgin females. (B) Functional inactivation of pLB1, but not of …
Figure 6—figure supplement 1
Inactivation of pLB1 cells phenocopies TβH mutant.
(A) Functional inactivation of pLB1 cells using the inwardly-rectifying K+ channel Kir (2.1b) that inhibits neuronal activity blocks egg laying in virgin females. (B) Ovaries of pLB1Gal4/UAS-TTX …
Figure 7
