Example spectra are shown that led to the identification of phosphorylated Ser13 m/z 1,456.732+ (A), Thr16 m/z 1,456.732+ (B), Thr19 m/z 1,991.962+ (C), Thr32 m/z 1,414.612+ (D), Ser92 m/z 1,902.872+ (E), Ser156 m/z 1,070.472+ (F), Ser156 and 158 2,243.062+ (G), Ser170and Ser178 m/z 1,926.812+ (H). The phosphopeptides recovered were _RDPAS(ph)PLTPLTPL_, _RDPASPLT(ph)PLTPL_, _RDPASPLTPLT(ph)PLSTEAF_, _TFEDVT(ph)PTGGVGR_, _SVFS(ph)PTSQSTLINGETR_, _PRS(ph)PTPSIH_ and _KIKVEDPRS(ph)PT(ph)PSIHHSR_. The ScaffoldPTM Viewer (version 3.0.0) was used to illustrate the spectra. The corresponding Ascore is indicated. An Ascore of 20 (p=0.01) should result in the site being localized with 99% certainty (Beausoleil et al., 2006).