(A) Schematic of Sox2 domains. HMG, High Mobility Group domain. TAD, Transactivation Domain. NLS, Nuclear Localization Signal. (B) Live-cell imaging of mouse ES cells stably expressing H2B-GFB and various Halo-tagged Sox2 truncations or mutations. Bottom, chromosome enrichment quantification for the various Halo-tagged Sox2 over-expressing (OE) constructs. Halo-Sox2 HMG construct is a truncation of Halo-Sox2 with the TAD region deleted. Halo-Sox2 TAD is a truncation of Halo-Sox2 with the HMG domain deleted. Halo-Sox2-DBD5M and Halo-Sox2 DBD3M are the full length Halo-Sox2 with 5 and 3 point mutations to abrogate DNA binding, respectively. Halo-Sox2 NLSM is the full length Halo-Sox2 with point mutations to abolish NLS function. n = 40 cells. (C) Live-cell imaging of mouse ES cells stably expressing H2B-GFP and HaloTag fused to SV40 NLS, plant-specific NLS, or by itself. Right, chromosome enrichment levels for the indicated HaloTag constructs. n = 40 cells. Data are represented as mean ± SEM. Scale bars, 5 µm.