sRNA-mediated activation of gene expression by inhibition of 5'-3’ exonucleolytic mRNA degradation
- UMR8261 CNRS - Université Paris Diderot, France
- Université de Strasbourg, CNRS, France
Abstract
Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can regulate the activity of these enzymes is an open question. We show that Bacillus subtilis RoxS, a major trans-acting sRNA shared with Staphylococus aureus, prevents degradation of the yflS mRNA, encoding a malate transporter. In the presence of malate, RoxS transiently escapes from repression by the NADH-sensitive transcription factor Rex and binds to the extreme 5’-end of yflS mRNA. This impairs the 5’-3’ exoribonuclease activity of RNase J1, increasing the half-life of the primary transcript and concomitantly enhances ribosome binding to increase expression of the transporter. Globally, the different targets regulated by RoxS suggest that it helps readjust the cellular NAD+/NADH balance when perturbed by different stimuli.
Article and author information
Author details
Funding
Université Paris Diderot (UMR8261)
- Ciarán Condon
Agence Nationale de la Recherche (ANR-10-LABX-0036 NETRNA)
- Pascale Romby
Agence Nationale de la Recherche (ANR-16-CE12-0002-01 BaRR)
- Sylvain Durand
Agence Nationale de la Recherche (ANR-12-BSV6-0007 asSUPYCO)
- Ciarán Condon
Centre National de la Recherche Scientifique (UMR8261,UPR9002)
- Sylvain Durand
- Frédérique Braun
- Anne-Catherine Helfer
- Pascale Romby
- Ciarán Condon
Université de Strasbourg (UPR9002)
- Pascale Romby
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Durand et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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sRNA-mediated activation of gene expression by inhibition of 5'-3’ exonucleolytic mRNA degradation
eLife 6:e23602.
https://doi.org/10.7554/eLife.23602
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