Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha
- Osaka University, Japan
- Universität Heidelberg, Germany
- University of Heidelberg, Germany
Abstract
Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood. Here we show that unlike the best-studied yeast Saccharomyces cerevisiae, position of pre-anaphase nucleus is not strongly biased toward bud neck in Ogataea polymorpha and the regulation of spindle positioning becomes active only shortly before anaphase. This is likely due to the unstable property of cMTs compared to those in S. cerevisiae. Furthermore, we show that cMT nucleation/anchoring is restricted at the level of recruitment of the γ-tubulin complex receptor, Spc72, to spindle pole body (SPB), which is regulated by the polo-like kinase Cdc5. Additionally, electron microscopy revealed that the cytoplasmic side of SPB is structurally different between G1 and anaphase. Thus, polo-like kinase dependent recruitment of γ-tubulin receptor to SPBs determines the timing of spindle orientation in O. polymorpha.
Data availability
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Ogataea polymorpha NCYC 495 leu1.1 v2.0The U.S. Department of Energy is committed to making its electronic and information technologies accessible to individuals with disabilities in accordance with Section 508 of the Rehabilitation Act (29 U.S.C. 794d), as amended in 1998.
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Opolymorpha_4329Publicly available at the DNA Data Bank of Japan (accession no. DF933569-DF933585).
Article and author information
Author details
Funding
Institute for Fermentation, Osaka (the Endowed Chair Program)
- Yoshinobu Kaneko
Japan Society for the Promotion of Science (JP24570214)
- Hiromi Maekawa
Deutsche Forschungsgemeinschaft (PE1883)
- Gislene Pereira
Deutsche Forschungsgemeinschaft (SFB873)
- Gislene Pereira
Deutsche Forschungsgemeinschaft (SFB1036)
- Gislene Pereira
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Anna Akhmanova, Utrecht University, Netherlands
Version history
- Received: December 19, 2016
- Accepted: August 17, 2017
- Accepted Manuscript published: August 30, 2017 (version 1)
- Version of Record published: October 3, 2017 (version 2)
Copyright
© 2017, Maekawa et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha
eLife 6:e24340.
https://doi.org/10.7554/eLife.24340
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