RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
- Max Planck Institute for Biophysical Chemistry, Germany
Abstract
For transcription through chromatin, RNA polymerase (Pol) II associates with elongation factors (EFs). Here we show that many EFs crosslink to RNA emerging from transcribing Pol II in the yeast Saccharomyces cerevisiae. Most EFs crosslink preferentially to mRNAs, rather than unstable non-coding RNAs. RNA contributes to chromatin association of many EFs, including the Pol II serine 2 kinases Ctk1 and Bur1 and the histone H3 methyltransferases Set1 and Set2. The Ctk1 kinase complex binds RNA in vitro, consistent with direct EF-RNA interaction. Set1 recruitment to genes in vivo depends on its RNA recognition motifs (RRMs). These results strongly suggest that nascent RNA contributes to EF recruitment to transcribing Pol II. We propose that EF-RNA interactions facilitate assembly of the elongation complex on transcribed genes when RNA emerges from Pol II, and that loss of EF-RNA interactions upon RNA cleavage at the polyadenylation site triggers disassembly of the elongation complex.
Data availability
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RNA contributes to chromatin association of transcription elongation factors and RNA polymerase II CTD kinasesPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE81822).
Article and author information
Author details
Funding
European Molecular Biology Organization (ALTF 745-2014)
- Seychelle M Vos
Center for Innovative Medicine
- Michael Lidschreiber
Science for Life Laboratory
- Michael Lidschreiber
Deutsche Forschungsgemeinschaft (SFB860 SPP1935)
- Patrick Cramer
European Research Council (693023)
- Patrick Cramer
Volkswagen Foundation
- Patrick Cramer
Max Planck Institute for Biophysical Chemistry (Open-access funding)
- Patrick Cramer
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Battaglia et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
eLife 6:e25637.
https://doi.org/10.7554/eLife.25637
