Glycolytic reliance promotes anabolism in photoreceptors
- Harvard Medical School, United States
Abstract
Vertebrate photoreceptors are among the most metabolically active cells, exhibiting a high rate of ATP consumption. This is coupled with a high anabolic demand, necessitated by the diurnal turnover of a specialized membrane-rich organelle, the outer segment, which is the primary site of phototransduction. How photoreceptors balance their catabolic and anabolic demands is poorly understood. Here, we show that rod photoreceptors in mice rely on glycolysis for their outer segment biogenesis. Genetic perturbations targeting allostery or key regulatory nodes in the glycolytic pathway impacted the size of the outer segments. Fibroblast growth factor signaling was found to regulate glycolysis, with antagonism of this pathway resulting in anabolic deficits. These data demonstrate the cell autonomous role of the glycolytic pathway in outer segment maintenance and provide evidence that aerobic glycolysis is part of a metabolic program that supports the biosynthetic needs of a normal neuronal cell type.
Article and author information
Author details
Funding
National Institutes of Health (R01 EY023291)
- Yashodhan Chinchore
- Tedi Begaj
- David Wu
- Eugene Drokhlyansky
- Constance L Cepko
Howard Hughes Medical Institute
- Constance L Cepko
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal care and use adhered to the Harvard Medical School's IACUC guidelines. Animals were handled in accordance with the protocol# 0428 and 04537.
Copyright
© 2017, Chinchore et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 3,794
- views
-
- 750
- downloads
-
- 158
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 158
- citations for umbrella DOI https://doi.org/10.7554/eLife.25946
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Glycolytic reliance promotes anabolism in photoreceptors
eLife 6:e25946.
https://doi.org/10.7554/eLife.25946
