Biofilm formation is critical for the infection cycle of Vibrio cholerae. Vibrio exopolysaccharides (VPS) and the matrix proteins RbmA, Bap1 and RbmC are required for the development of biofilm architecture. We demonstrate that RbmA binds VPS directly and uses a binary structural switch within its first fibronectin type III (FnIII-1) domain to control RbmA structural dynamics and the formation of VPS-dependent higher-order structures. The structural switch in FnIII-1 regulates interactions in trans with the FnIII-2 domain, leading to open (monomeric) or closed (dimeric) interfaces. The ability of RbmA to switch between open and closed states is important for V. cholerae biofilm formation, as RbmA variants with switches that are locked in either of the two states lead to biofilms with altered architecture and structural integrity.
- Fitnat H Yildiz
- Carrie L Partch
- Knut Drescher
- Alicia K Michael
- Lars EP Dietrich
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Dianne K Newman, Howard Hughes Medical Institute, California Institute of Technology, United States
© 2017, Fong et al.
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