Sec17/Sec18 act twice, enhancing membrane fusion and then disassembling cis-SNARE complexes
- Geisel School of Medicine at Dartmouth, United States
- University of Washington, United States
Abstract
At physiological protein levels, the slow HOPS- and SNARE-dependent fusion which occurs upon complete SNARE zippering is stimulated by Sec17 and Sec18:ATP without requiring ATP hydrolysis. To stimulate, Sec17 needs its central residues which bind the 0-layer of the SNARE complex and its N-terminal apolar loop. Adding a transmembrane anchor to the N-terminus of Sec17 bypasses this requirement for apolarity of the Sec17 loop, suggesting that the loop functions for membrane binding rather than to trigger bilayer rearrangement. In contrast, when complete C-terminal SNARE zippering is prevented, fusion strictly requires Sec18 and Sec17, and the Sec17 apolar loop has functions beyond membrane anchoring. Thus Sec17 and Sec18 act twice in the fusion cycle, binding to trans-SNARE complexes to accelerate fusion, then hydrolyzing ATP to disassemble cis-SNARE complexes.
Article and author information
Author details
Funding
National Institutes of Health (R01 GM23377-40)
- William T Wickner
National Institutes of Health (R35GM118037-01)
- William T Wickner
National Institutes of Health (GM077349)
- Alexey J Merz
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Song et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Sec17/Sec18 act twice, enhancing membrane fusion and then disassembling cis-SNARE complexes
eLife 6:e26646.
https://doi.org/10.7554/eLife.26646
