Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6
Abstract
Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily tracked in a mixed population of cells. We describe a method to multiplex homologous recombination (HR) in human hematopoietic stem and progenitor cells and primary human T cells by combining rAAV6 donor delivery and the CRISPR/Cas9 system delivered as ribonucleoproteins (RNPs). In addition, the use of reporter genes allows FACS-purification and tracking of cells that have had multiple alleles or loci modified by HR. We believe this method will enable broad applications not only to the study of human hematopoietic gene function and networks, but also to perform sophisticated synthetic biology to develop innovative engineered stem cell-based therapeutics.
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Funding
Danish Council for Independent Research (DFF-1333-00106B)
- Rasmus O Bak
Danish Council for Independent Research (DFF-1331-00735B)
- Rasmus O Bak
National Institutes of Health (R01- AI097320)
- Matthew H Porteus
National Institutes of Health (R01-AI120766)
- Matthew H Porteus
Stanford Child Health Research Institute (Postdoctoral Award)
- Daniel P Dever
Austrian Research Council (Erwin Schroedinger Postdoctoral Fellowship)
- Andreas Reinisch
Amon G. Carter Foundation
- Matthew H Porteus
Laurie Kraus Lacob Faculty Scholar Award in Pediatric Translational Research (Scholar Award)
- Matthew H Porteus
National Institutes of Health (PN2EY018244)
- Matthew H Porteus
Stanford Ludwig Center for Cancer Stem Cell Research
- Ravindra Majeti
National Institutes of Health (R01-CA188055)
- Ravindra Majeti
New York Stem Cell Foundation (Robertson Investigator)
- Ravindra Majeti
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal experiments were performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The experimental protocol was approved by Stanford University's Administrative Panel on Lab Animal Care (IACUC 25065).
Copyright
© 2017, Bak et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Further reading
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