High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
- University of Washington, United States
- Duke University Medical Center, United States
Abstract
In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.
Data availability
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High-resolution mapping of heteroduplex DNA formed in UV-induced and spontaneous mitotic recombination events in yeastPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE100497).
Article and author information
Author details
Funding
National Institute of General Medical Sciences (GM24110)
- Yi Yin
- Margaret Dominska
- Eunice Yim
- Thomas D Petes
National Institute of General Medical Sciences (GM52319)
- Yi Yin
- Margaret Dominska
- Eunice Yim
- Thomas D Petes
National Institute of General Medical Sciences (1R35GM118020)
- Yi Yin
- Margaret Dominska
- Eunice Yim
- Thomas D Petes
National Institute of General Medical Sciences (5T32GM007754-37)
- Yi Yin
- Margaret Dominska
- Eunice Yim
- Thomas D Petes
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Yin et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
eLife 6:e28069.
https://doi.org/10.7554/eLife.28069
