(A) Cartoon depicting truncated regions in our CaV2.1 α1 deletion mutants including the binding sites for CaVβ4, CASK, RBP, PXXP, RIM1/2 and Mint-1 along with the effects of C-terminal truncations on ICa. (B) Averaged traces of RRP and total releasable pool measurements from mice expressing Cre + full transcript CaV2.1 rescue (grey), Δ2365–2368 (cyan), Δ2213–2368 (yellow), Δ2061–2368 (purple), Δ2042–2368 (green) or Δ2016–2368 (blue). ICa (top) and EPSCs (bottom) triggered by 3 ms and 30 ms pulses, plotted on top of each other (n = 10 for each group, except for Δ2212–2368: n = 8). (C–H) Quantification of ICa charge (3 ms: C; and 30 ms: F), max. EPSC amplitudes (3 ms: D; 30 ms: G) and the 10–90% rise of the EPSCs (3 ms: E; 30 ms: H). All data are depicted as mean ± SEM. Detailed values can be derived from Table 2.