Sumoylation promotes optimal APC/C activation and timely anaphase
- Johns Hopkins University, United States
- Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, United States
Abstract
The Anaphase Promoting Complex/Cyclosome (APC/C) is a ubiquitin E3 ligase that functions as the gatekeeper to mitotic exit. APC/C activity is controlled by an interplay of multiple pathways during mitosis, including the spindle assembly checkpoint (SAC), that are not yet fully understood. Here, we show that sumoylation of the APC4 subunit of the APC/C peaks during mitosis and is critical for timely APC/C activation and anaphase onset. We have also identified a functionally important SUMO interacting motif in the cullin-homology domain of APC2 located near the APC4 sumoylation sites and APC/C catalytic core. Our findings provide evidence of an important regulatory role for SUMO modification and binding in affecting APC/C activation and mitotic exit.
Article and author information
Author details
Funding
National Institutes of Health (T32 CA009110)
- Christine C Lee
Howard Hughes Medical Institute
- Hongtao Yu
Cancer Prevention and Research Institute of Texas (RP120717-P2)
- Hongtao Yu
Welch Foundation (I-1441)
- Hongtao Yu
National Institutes of Health (GM060980)
- Michael J Matunis
Cancer Prevention and Research Institute of Texas (RP160667-P2)
- Hongtao Yu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Lee et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Sumoylation promotes optimal APC/C activation and timely anaphase
eLife 7:e29539.
https://doi.org/10.7554/eLife.29539
