(A) rnts>egr, CtBPQ229*/+ wing disc with randomly-generated clones (two independent FLP-out constructs, Act<stop<lacZnls and Ubi<stop< GFPnls) generated with a 10 min heat-shock (37°C) at the start of the temperature shift from 18o C to 30o C and dissected 72 hr after the downshift. In (A’), there are three uniquely labeled clones (only Ubi-GFP, only Act-lacZ, or both), as indicated by arrowheads of matching color, in the ectopic pouch. (B–C) Lineage tracing following rnts>egr induced damage in wild type (B) and CtBPQ229*/+ (C) genetic backgrounds with R76B02-lexA, lexAOp-FLP, Ubi<stop<GFPnls after 72 hr of recovery. (D–F) rnts>egr, CtBPQ229*/+ wing discs with the enhancer trap mirr-lacZ stained with anti-Wg. (D) Undamaged. (E–F) Damaged discs, after 0 hr (E) and 72 hr (F) of recovery. N indicates notum, H indicates hinge. The asterisk indicates area of increased Wg expression in (E) and the ectopic wing pouch in (F). (G) Frequency of ectopic wings (EW) following rnts>egr damage in wild type or CtBP334Δ4/+ together with different mirr alleles, mirr1486/+ and mirr1825/+. Error bars show standard deviations. (H–J) rnts>egr, CtBPQ229*/+wing discs at different time points of recovery with dilp8-GFP, anti-Cubitus Interruptus (Ci) for marking the anterior compartment and anti-Engrailed (En) for marking the posterior compartment. Note that dilp8-GFP expression initiates in the posterior compartment and then spreads to the anterior compartment. (K) rnts>egr, CtBPQ229*/+ wing discs containing randomly generated marked clones (similar to A) that were induced on day 5 (48 hr before the temperature shift). Note the large GFP-positive clone in the posterior compartment of the ectopic pouch that abuts a significant portion of the anterior/posterior compartment boundary. Dotted boxes indicate areas that are enlarged in subsequent panels. The time of recovery (i.e. after downshift) is indicated in the bottom right corner of the relevant panel.