(A) NKG2D surface levels measured by flow cytometry of blood NK cells 48 hr after injection of blocking antibody specific for the indicated NKG2D ligand. Data are representative of >4 independent …
(A) Specific blockade of NKG2D binding by anti-RAE-1 mAbs. The indicated cells lines were incubated for 20 min at 4°C with blocking antibody. Subsequently and without washing, biotinylated NKG2D-Fc …
(A) NKG2D surface levels on NK cells from bone marrow and liver. Data are representative of two independent experiments. Statistical significance was determined using two-tailed unpaired Student’s t …
(A) WT or RAE-1-KO splenic NK cell IFNγ production and degranulation (CD107a) after 5 hr ex vivo stimulation with platebound control Ig or anti-NKp46. (B and C) Percentage of activated (IFNγ- and …
(A, left panel) NK cells from WT and RAE-1-KO mice as percentage of live splenocytes. Data are representative of >4 independent experiments. (A, right panel) CD27 and CD11b
expression on splenic NK cells from WT and RAE-1-KO mice. Data are representative of three independent experiments. (B) NK cells from WT, RAE-1-KO, or NKG2D-KO mice (n = 3) were pre-activated with a single injection of 200 g Poly I:C. Two days later, peritoneal wash cells were harvested, pooled, and used as effector cells to kill YAC-1 cells in a standard 51Cr in vitro cytotoxicity assay, at the indicated effector: target ratios. Data are representative of two independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t tests (A) or two-way ANOVA (B). Data represent means ± SEM (error bars are not visible because they are small).
(A and B) Percentage of activated splenic or peritoneal NK cells from WT or NKG2D-KO mice after ex vivo stimulation. Data are representative of >4 independent experiments. (C) NK activation in WT, …
(A) NK activation in WT, RAE-1-KO, and NKG2D-KO peritoneal cells after ex vivo stimulation with platebound anti-NKG2D antibody. Data are representative of three independent experiments. (B) …
(A) NKG2D cell surface levels on blood NK cells 8 weeks after WT or RAE-1-KO mice were lethally irradiated and reconstituted with WT or RAE-1-KO bone marrow. Data are representative of three …
(A) NKG2D surface levels on NK cells from spleens or lymph nodes of RAE-1-KO chimeric mice. Data are representative of three independent experiments. (B) Gating strategy for the four populations of …
RAE-1ε MFI levels on endothelial cells (CD45-neg; Ter119-neg; CD31+) in the indicated tissues.
(A) NKG2D surface levels on NK cells infiltrating subcutaneous B16 tumors in mice 48 hr after injection of the indicated antibody. Data are representative of three independent experiments. (B) NKG2D …
(A) Repetition of the experiment in Figure 5C. Statistical significance was determined using one-way ANOVA with Bonferroni post-tests. (B) RAE-1ε staining on endothelial cells in established B16 or …
(A) WT or NKG2D-KO mice (n = 29–30) were challenged with 2 × 104 B16 cells i.v. and monitored for morbidity. Matched groups of each strain (n = 9–10) were depleted of NK cells before implanting the …
(A) Rag2-KO NKG2D-WT or Rag2-KO NKG2D-KO mice were challenged with 1 × 104 B16 cells s.c. and monitored for tumor growth. Data are representative of three independent experiments. (B) Rag2-KO …