(A, left panel) NK cells from WT and RAE-1-KO mice as percentage of live splenocytes. Data are representative of >4 independent experiments. (A, right panel) CD27 and CD11b
expression on splenic NK cells from WT and RAE-1-KO mice. Data are representative of three independent experiments. (B) NK cells from WT, RAE-1-KO, or NKG2D-KO mice (n = 3) were pre-activated with a single injection of 200 g Poly I:C. Two days later, peritoneal wash cells were harvested, pooled, and used as effector cells to kill YAC-1 cells in a standard 51Cr in vitro cytotoxicity assay, at the indicated effector: target ratios. Data are representative of two independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t tests (A) or two-way ANOVA (B). Data represent means ± SEM (error bars are not visible because they are small).