Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle
- State University of New York, Upstate Medical University, United States
- Syracuse University, United States
Abstract
How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer's vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.
Article and author information
Author details
Jonathan Edward Dawson
Funding
National Institutes of Health (R01HL095690)
- Jeffrey D Amack
National Institutes of Health (R01GM117598)
- M Lisa Manning
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Marianne Bronner, California Institute of Technology, United States
Version history
- Received: August 5, 2017
- Accepted: January 26, 2018
- Accepted Manuscript published: January 29, 2018 (version 1)
- Version of Record published: February 6, 2018 (version 2)
Copyright
© 2018, Dasgupta et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle
eLife 7:e30963.
https://doi.org/10.7554/eLife.30963
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