1. Cell Biology
  2. Microbiology and Infectious Disease
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Coupling chemosensory array formation and localization

  1. Alejandra Alvarado
  2. Andreas Kjær
  3. Wen Yang
  4. Petra Mann
  5. Ariane Briegel
  6. Matthew K Waldor
  7. Simon Ringgaard  Is a corresponding author
  1. Max Planck Institute for Terrestrial Microbiology, Germany
  2. Leiden University, Netherlands
  3. Brigham and Women's Hospital, United States
Research Article
  • Cited 13
  • Views 1,496
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Cite this article as: eLife 2017;6:e31058 doi: 10.7554/eLife.31058

Abstract

Chemotaxis proteins organize into large, highly ordered, chemotactic signaling arrays, which in Vibrio species are found at the cell pole. Proper localization of signaling arrays is mediated by ParP, which tethers arrays to a cell pole anchor, ParC. Here we show that ParP's C-terminus integrates into the core-unit of signaling arrays through interactions with MCP-proteins and CheA. Its intercalation within core-units stimulates array formation, whereas its N-terminal interaction domain enables polar recruitment of arrays and facilitates its own polar localization. Linkage of these domains within ParP couples array formation and localization and results in controlled array positioning at the cell pole. Notably, ParP's integration into arrays modifies its own and ParC's subcellular localization dynamics, promoting their polar retention. ParP serves as a critical nexus that regulates the localization dynamics of its network constituents and drives the localized assembly and stability of the chemotactic machinery, resulting in proper cell pole development.

Article and author information

Author details

  1. Alejandra Alvarado

    Department for Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  2. Andreas Kjær

    Department for Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Wen Yang

    Institute of Biology, Leiden University, Leiden, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  4. Petra Mann

    Department for Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  5. Ariane Briegel

    Institute of Biology, Leiden University, Leiden, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  6. Matthew K Waldor

    Division of Infectious Diseases, Brigham and Women's Hospital, Boston, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1843-7000
  7. Simon Ringgaard

    Department for Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
    For correspondence
    simon.ringgaard@mpi-marburg.mpg.de
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4980-5964

Funding

Max-Planck-Institut für Terrestrische Mikrobiologie (Open-access funding)

  • Simon Ringgaard

Deutsche Forschungsgemeinschaft (RI 2820/1-1)

  • Simon Ringgaard

National Institutes of Health (NIH R37 AI-042347)

  • Matthew K Waldor

Howard Hughes Medical Institute

  • Matthew K Waldor

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Tâm Mignot, Aix Marseille University-CNRS UMR7283, France

Publication history

  1. Received: August 5, 2017
  2. Accepted: October 22, 2017
  3. Accepted Manuscript published: October 23, 2017 (version 1)
  4. Version of Record published: November 29, 2017 (version 2)

Copyright

© 2017, Alvarado et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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