Coupling chemosensory array formation and localization
- Max Planck Institute for Terrestrial Microbiology, Germany
- Leiden University, Netherlands
- Brigham and Women's Hospital, United States
Abstract
Chemotaxis proteins organize into large, highly ordered, chemotactic signaling arrays, which in Vibrio species are found at the cell pole. Proper localization of signaling arrays is mediated by ParP, which tethers arrays to a cell pole anchor, ParC. Here we show that ParP's C-terminus integrates into the core-unit of signaling arrays through interactions with MCP-proteins and CheA. Its intercalation within core-units stimulates array formation, whereas its N-terminal interaction domain enables polar recruitment of arrays and facilitates its own polar localization. Linkage of these domains within ParP couples array formation and localization and results in controlled array positioning at the cell pole. Notably, ParP's integration into arrays modifies its own and ParC's subcellular localization dynamics, promoting their polar retention. ParP serves as a critical nexus that regulates the localization dynamics of its network constituents and drives the localized assembly and stability of the chemotactic machinery, resulting in proper cell pole development.
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Funding
Max-Planck-Institut für Terrestrische Mikrobiologie (Open-access funding)
- Simon Ringgaard
Deutsche Forschungsgemeinschaft (RI 2820/1-1)
- Simon Ringgaard
National Institutes of Health (NIH R37 AI-042347)
- Matthew K Waldor
Howard Hughes Medical Institute
- Matthew K Waldor
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Alvarado et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Coupling chemosensory array formation and localization
eLife 6:e31058.
https://doi.org/10.7554/eLife.31058
