1. Neuroscience
Download icon

Phase-tuned neuronal firing encodes human contextual representations for navigational goals

  1. Andrew J Watrous  Is a corresponding author
  2. Jonathan Miller
  3. Salman E Qasim
  4. Itzhak Fried
  5. Joshua Jacobs
  1. Columbia University, United States
  2. University of California, Los Angeles, United States
Research Advance
  • Cited 24
  • Views 2,278
  • Annotations
Cite this article as: eLife 2018;7:e32554 doi: 10.7554/eLife.32554

Abstract

We previously demonstrated that the phase of oscillations modulates neural activity representing categorical information using human intracranial recordings and high-frequency activity from local field potentials (Watrous et al., 2015b). We extend these findings here using human single-neuron recordings during a navigation task. We identify neurons in the medial temporal lobe with firing-rate modulations for specific navigational goals, as well as for navigational planning and goal arrival. Going beyond this work, using a novel oscillation detection algorithm, we identify phase-locked neural firing that encodes information about a person's prospective navigational goal in the absence of firing rate changes. These results provide evidence for navigational planning and contextual accounts of human MTL function at the single-neuron level. More generally, our findings identify phase-coded neuronal firing as a component of the human neural code.

Article and author information

Author details

  1. Andrew J Watrous

    Department of Biomedical Engineering, Columbia University, New York, United States
    For correspondence
    andrew.j.watrous@gmail.com
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3107-3726
  2. Jonathan Miller

    Department of Biomedical Engineering, Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Salman E Qasim

    Department of Biomedical Engineering, Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Itzhak Fried

    Department of Neurosurgery, University of California, Los Angeles, Los Angeles, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5962-2678
  5. Joshua Jacobs

    Department of Biomedical Engineering, Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.

Funding

National Institute of Neurological Disorders and Stroke (NS033221)

  • Itzhak Fried

National Institute of Neurological Disorders and Stroke (NS084017)

  • Itzhak Fried

National Institute of Mental Health (MH104606)

  • Joshua Jacobs

National Science Foundation (DGE 16-44869)

  • Salman E Qasim

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Human subjects: The Medical Institutional Review Board at the University of California-Los Angeles approved this study (IRB#10-000973) involving recordings from patients with drug-resistant epilepsy who provided informed consent to participate in research.

Reviewing Editor

  1. Timothy E Behrens, University of Oxford, United Kingdom

Publication history

  1. Received: October 12, 2017
  2. Accepted: June 21, 2018
  3. Accepted Manuscript published: June 22, 2018 (version 1)
  4. Version of Record published: July 11, 2018 (version 2)

Copyright

© 2018, Watrous et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,278
    Page views
  • 365
    Downloads
  • 24
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

  1. Further reading

Further reading

    1. Neuroscience
    Julio D Perez et al.
    Tools and Resources Updated

    Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using celltype-specific metabolic labeling of isolated neurites, we demonstrated that the processes of glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation are general properties of neurons.

    1. Neuroscience
    Yunbo Li et al.
    Research Article

    The conserved MAP3K Dual leucine zipper kinases can activate JNK via MKK4 or MKK7. Vertebrate DLK and LZK share similar biochemical activities and undergo auto-activation upon increased expression. Depending on cell-type and nature of insults DLK and LZK can induce pro-regenerative, pro-apoptotic or pro-degenerative responses, although the mechanistic basis of their action is not well understood. Here, we investigated these two MAP3Ks in cerebellar Purkinje cells using loss- and gain-of function mouse models. While loss of each or both kinases does not cause discernible defects in Purkinje cells, activating DLK causes rapid death and activating LZK leads to slow degeneration. Each kinase induces JNK activation and caspase-mediated apoptosis independent of each other. Significantly, deleting CELF2, which regulates alternative splicing of Map2k7, strongly attenuates Purkinje cell degeneration induced by LZK, but not DLK. Thus, controlling the activity levels of DLK and LZK is critical for neuronal survival and health.