(A) Quantification of mitochondrial Ca2+ using animals expressing mito::GCaMP6 and mCherry in their body wall muscle (takEx347). (B) Quantification of ALM neuronal mitochondrial morphology. Analysis was done using transgenic animals expressing mito::GFP in mechanosensory neurons (jsIs609). Data are displayed with blue and green representing the percentage of animals displaying continuous and discontinuous mitochondrial morphology, respectively. **p<0.01, ***p<0.001 (Chi-square test). (C) H2DCF-DA assay measuring ROS levels relative to wild type. Data are from three replicate assays. (D) Basal and (E) maximal respiration rates (after exposure to FCCP) of wild type, sel-12(ty11), and mcu-1;sel-12(ty11) animals. Data are from three replicate assays. (F) Quantification of aberrant neuronal structures (sprouts/branches) in ALM neurons in day one animals. ALM neuronal morphology analysis is done using transgenic animals expressing mec-4p::GFP (zdIs5). (G) Quantification of the response of day one wild type, sel-12(ty11), mcu-1;sel-12(ty11) and mcu-1 animals to light anterior and posterior touch. (H) ATP and (I) ROS levels normalized to protein content in skin fibroblasts isolated from control and FAD patients, treated with Ru360. n = number of animals analyzed per genotype. Data are displayed as mean ± SEM, and all comparisons have been made to wild type animals unless otherwise indicated). ns p>0.05, ***p<0.0001 (One way ANOVA with Tukey test).