A sleep state in Drosophila larvae required for neural stem cell proliferation
Abstract
Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.
Article and author information
Author details
Funding
National Institutes of Health (K08NS090461)
- Matthew S Kayser
Burroughs Wellcome Fund
- Matthew S Kayser
Alfred P. Sloan Foundation
- Matthew S Kayser
March of Dimes Foundation
- Matthew S Kayser
National Institutes of Health (R01NS088432)
- David M Raizen
National Institutes of Health (R01NS084835)
- Christopher Fang-Yen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Mani Ramaswami, Trinity College Dublin, Ireland
Version history
- Received: October 30, 2017
- Accepted: February 8, 2018
- Accepted Manuscript published: February 9, 2018 (version 1)
- Version of Record published: March 2, 2018 (version 2)
Copyright
© 2018, Szuperak et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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