A sleep state in Drosophila larvae required for neural stem cell proliferation
- University of Pennsylvania, United States
Abstract
Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.
Article and author information
Author details
Christopher Fang-Yen
Funding
National Institutes of Health (K08NS090461)
- Matthew S Kayser
Burroughs Wellcome Fund
- Matthew S Kayser
Alfred P. Sloan Foundation
- Matthew S Kayser
March of Dimes Foundation
- Matthew S Kayser
National Institutes of Health (R01NS088432)
- David M Raizen
National Institutes of Health (R01NS084835)
- Christopher Fang-Yen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Szuperak et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A sleep state in Drosophila larvae required for neural stem cell proliferation
eLife 7:e33220.
https://doi.org/10.7554/eLife.33220
