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A sleep state in Drosophila larvae required for neural stem cell proliferation

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Cite this article as: eLife 2018;7:e33220 doi: 10.7554/eLife.33220

Abstract

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.

Article and author information

Author details

  1. Milan Szuperak

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Matthew A Churgin

    Department of Bioengineering, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2299-0124
  3. Austin J Borja

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. David M Raizen

    Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5935-0476
  5. Christopher Fang-Yen

    Department of Bioengineering, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4568-3218
  6. Matthew S Kayser

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    For correspondence
    kayser@pennmedicine.upenn.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2359-4967

Funding

National Institutes of Health (K08NS090461)

  • Matthew S Kayser

Burroughs Wellcome Fund

  • Matthew S Kayser

Alfred P. Sloan Foundation

  • Matthew S Kayser

March of Dimes Foundation

  • Matthew S Kayser

National Institutes of Health (R01NS088432)

  • David M Raizen

National Institutes of Health (R01NS084835)

  • Christopher Fang-Yen

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Mani Ramaswami, Trinity College Dublin, Ireland

Publication history

  1. Received: October 30, 2017
  2. Accepted: February 8, 2018
  3. Accepted Manuscript published: February 9, 2018 (version 1)
  4. Version of Record published: March 2, 2018 (version 2)

Copyright

© 2018, Szuperak et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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