1. Neuroscience
Download icon

A sleep state in Drosophila larvae required for neural stem cell proliferation

Research Article
  • Cited 0
  • Views 1,098
  • Annotations
Cite as: eLife 2018;7:e33220 doi: 10.7554/eLife.33220

Abstract

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.

Article and author information

Author details

  1. Milan Szuperak

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Matthew A Churgin

    Department of Bioengineering, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-2299-0124
  3. Austin J Borja

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. David M Raizen

    Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0001-5935-0476
  5. Christopher Fang-Yen

    Department of Bioengineering, University of Pennsylvania, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-4568-3218
  6. Matthew S Kayser

    Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    For correspondence
    kayser@pennmedicine.upenn.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-2359-4967

Funding

National Institutes of Health (K08NS090461)

  • Matthew S Kayser

Burroughs Wellcome Fund

  • Matthew S Kayser

Alfred P. Sloan Foundation

  • Matthew S Kayser

March of Dimes Foundation

  • Matthew S Kayser

National Institutes of Health (R01NS088432)

  • David M Raizen

National Institutes of Health (R01NS084835)

  • Christopher Fang-Yen

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Mani Ramaswami, Reviewing Editor, Trinity College Dublin, Ireland

Publication history

  1. Received: October 30, 2017
  2. Accepted: February 8, 2018
  3. Accepted Manuscript published: February 9, 2018 (version 1)

Copyright

© 2018, Szuperak et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,098
    Page views
  • 208
    Downloads
  • 0
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)