Multiple inputs ensure yeast cell size homeostasis during cell cycle progression

  1. Cecilia Garmendia-Torres
  2. Olivier Tassy
  3. Audrey Matifas
  4. Nacho Molina
  5. Gilles Charvin  Is a corresponding author
  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, France

Abstract

Coordination of cell growth with division is essential for proper cell function. In budding yeast, although some molecular mechanisms responsible for cell size control during G1 have been elucidated, the mechanism by which cell size homeostasis is established remains to be discovered. Here, we developed a new technique based on quantification of histone levels to monitor cell cycle progression in individual cells with unprecedented accuracy. Our analysis establishes the existence of a mechanism controlling bud size in G2/M that prevents premature onset of anaphase, and controls the overall size variability. While most G1 mutants do not display impaired size homeostasis, mutants in which Cyclin B-Cdk regulation is altered display large size variability. Our study thus demonstrates that size homeostasis is not controlled by a G1-specific mechanism alone but is likely to be an emergent property resulting from the integration of several mechanisms that coordinate cell and bud growth with division.

Data availability

The raw cell cycle data are available on a dedicated server http://charvin.igbmc.science/yeastcycledynamics/ and further details on how to use the site are available in Appendix 1. The autotrack software is available at GitHub: https://github.com/gcharvin/autotrack

The following data sets were generated

Article and author information

Author details

  1. Cecilia Garmendia-Torres

    Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Olivier Tassy

    Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Audrey Matifas

    Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Nacho Molina

    Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Gilles Charvin

    Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    For correspondence
    charvin@igbmc.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6852-6952

Funding

Atip-avenir CNRS/Inserm

  • Gilles Charvin

Agence Nationale de la Recherche (ANR-10-LABX-0030-INRT)

  • Gilles Charvin

Agence Nationale de la Recherche (ANR-10-IDEX-0002-02)

  • Gilles Charvin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Andrea Musacchio, Max Planck Institute of Molecular Physiology, Germany

Publication history

  1. Received: December 2, 2017
  2. Accepted: July 1, 2018
  3. Accepted Manuscript published: July 4, 2018 (version 1)
  4. Version of Record published: August 9, 2018 (version 2)
  5. Version of Record updated: September 24, 2019 (version 3)

Copyright

© 2018, Garmendia-Torres et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Cecilia Garmendia-Torres
  2. Olivier Tassy
  3. Audrey Matifas
  4. Nacho Molina
  5. Gilles Charvin
(2018)
Multiple inputs ensure yeast cell size homeostasis during cell cycle progression
eLife 7:e34025.
https://doi.org/10.7554/eLife.34025
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