(a) The translation blocking MO (Dkk2MO) targets the initiation codon. (b) Western blot using lysates from embryos injected with Dkk2-Flag mRNA (10 ng) alone or in combination with increasing … see more
Quantification of Dkk2 knockdown phenotype.
(a) Schematic representation of the dkk2 locus. The position of the splice blocking MO (Dkk2SMO) is shown (red line). The PCR primers used to analyze the spliced (E1/E2) and unspliced (I2/I3) … see more
Injection of Xenopus Dkk2 plasmid DNA (10 pg) restores snai2 expression in Dkk2SMO-injected (30 ng) embryos. Results from two independent experiments. For the phenotype of Dkk2 gain of function see F… see more
(a). Unilateral injection of Dkk2SMO (20 ng) did not affect the expression levels of pax3, sox8 and snai1, although their expression was shifted laterally. (b) The neural crest specifier twist1 was … see more
(a) At the 2 cell stage, mRNAs encoding noggin (50 pg) and wnt8 (100 pg) were injected in the animal pole region alone or in combination with Dkk2SMO (30 ng) or CoMO (30 ng). At the blastula stage … see more
(a) Temporal expression of dkk2.L and dkk2.S by qRT-PCR. (b) By in situ hybridization, at all stages examined (NF stage 11-33/34) dkk2 does not appear to be spatially restricted. Sense probe is … see more
(a) Unilateral injection of Dkk2SMO (30 ng) reduced snai2 expression. This phenotype was efficiently rescued by injection of plasmid DNA encoding lrp6 (50 pg) or β-catenin (50 pg), and to a lesser … see more
Quantification of Dkk2 knockdown phenotype upon β-catenin, lrp6 or wnt8 coexpression.
(a) Unilateral injection of dkk2 mRNA (500 pg) or dkk2 plasmid DNA (25 pg) expanded snai2 expression domain laterally. (b) Quantification of the Dkk2 overexpression phenotype. The number of embryos … see more
Quantification of Dkk2 gain-of-function phenotype.
(a) Unilateral injection of dkk2 plasmid DNA (50 pg) expanded snai2 expression domain laterally (arrowhead). This activity was blocked in the context of β-catenin- (β-catMO; 20 ng), Lrp6- (Lrp6MO; … see more
Quantification of β-catenin, Lrp6 and Wnt8 knockdown phenotypes upon Dkk2 coexpression.
(a) BIO treatment (10 μM) expanded sox10 expression domain anteriorly. Unilateral injection of Dkk2SMO (30 ng) reduced sox10 expression a phenotype that cannot be rescued by BIO treatment. In … see more
Quantification of Dkk2 knockdown and Dkk1 overexpression phenotypes upon BIO treatment.
During neural crest induction, Lrp6 mediates two independent signaling events triggered by Wnt8 and Dkk2 converging on β-catenin to promote neural crest formation (snai2 induction). The Lrp6/Wnt/Fzd … see more
(a) Ventral injection of wnt8 mRNA (50 pg) in the equatorial region of 4 cell stage embryos resulted in secondary dorsal axis formation at stage 30. Injection of Dkk2SMO (30 ng) significantly … see more