Evolution: Oxygen and early animals
Once upon a time – between 800 and 550 million years ago – the increasing levels of oxygen in the environment enabled animals to start evolving into the diverse life-forms that inhabit the Earth today (Lyons et al., 2014). Energetically speaking, life with oxygen is better than life without: a given amount of glucose processed in the presence of oxygen produces 18 times as much energy as the same amount of glucose processed without oxygen. Nevertheless, the use of oxygen also poses risks, and fluctuations in oxygen levels can lead to some of the toxic byproducts of metabolism accumulating in cells. Hence, animals have evolved elegant mechanisms to monitor oxygen levels and respond to changes.
In all animals studied so far, a specific set of molecules known as the HIF pathway has a central role in these mechanisms. When oxygen levels are low, this pathway – named after a protein called the hypoxia-inducible factor – triggers changes that help to maintain vital physiological functions in the cell (Bishop and Ratcliffe, 2014). Under normal oxygen conditions, an enzyme called prolyl hydroxylase modifies a particular amino acid (proline) in the HIF protein, which initiates its degradation via a protein called the von Hippel-Lindau protein. However, when oxygen levels are low, HIF remains intact and activates genes involved in anaerobic metabolism or processes that support oxygen delivery.
The HIF pathway has been found in the phylum Placozoa, which are among the simplest of all animal phyla (Loenarz et al., 2011), but until now it has not been clear if the ability to sense oxygen was present in the earliest animals, or if it evolved later. Now, in eLife, Gert Wörheide and colleagues at the Ludwig-Maximillians-Universität München and the University of Southern Denmark – including Daniel Mills and Warren Francis as joint first authors – report that early animals could have thrived without this pathway and survived with just a hint of oxygen (Mills et al., 2018).
The researchers decided to compare the first two lineages to have split from all other animals: sponges and comb jellies (Figure 1). Both phyla seem to survive extended periods of low oxygen, whereas the placozoans do not (Mills et al., 2014; Purcell et al., 2001; Loenarz et al., 2011). And since the oxygen levels in the ocean only increased about 200 million years after the first animals started to diverge, it is possible that animals had already started to evolve during a time when oxygen was scarce (Lyons et al., 2014; Dohrmann and Wörheide, 2017; Parfrey et al., 2011).
By comparing the genomes of several species of sponges and comb jellies, Mills et al. were able to show that they lack key gene components of the HIF pathway. This suggests that this pathway evolved after the last common ancestor of all living animals split from sponges and comb jellies and, therefore, it is not a universal trait of all modern animals.
Mills et al. make a convincing case for the absence of the HIF pathway in sponges: every sponge lacked most of the key components of the pathway. However, one of the comb jellies had a proline sequence in the HIF that was similar to sequences found in other animals. This could mean that the ancestors of comb jellies may have had an HIF-like pathway, but subsequently lost it at some point during evolution. The order in which the sponges and the comb jellies diverged from the other animals is currently debated (Feuda et al., 2017; King and Rokas, 2017). Evidence from anatomical and phylogenetic studies suggests that the sponges split first. However, if the comb jellies diverged first, it would challenge the idea that HIF was not present in the earliest animals.
Next, Mills et al. tested if the lack of an HIF pathway also led to the absence of (transcriptional) changes in gene activity when oxygen was scarce. When the sponge Tethya wilhelma was exposed to very low levels of oxygen (just 0.25% of modern levels), only a few genes changed over the course of four days of observation. Moreover, genes related to metabolism and stress did not change. However, when T. wilhelma was completely deprived of oxygen for just one hour, thousands of genes were activated, including several metabolic genes and a number of genes related to stress.
So, how can sponges respond to low oxygen conditions if they lack a functional HIF pathway? This pathway regulates the transcription of genes to make messenger RNA (mRNA), but it does not directly regulate the translation of mRNA to make proteins. There are, however, several oxygen dependent mechanisms that sponges could use to regulate translation: some bacteria, for example, use prolyl hydroxylases to target the components that produce proteins, while humans use specified gene duplicates to initiate protein production (Scotti et al., 2014; Uniacke et al., 2012).
Alternatively, sponges might use a different approach to respond to low levels of oxygen. One classic role of the HIF pathway is to redirect energy production away from aerobic reactions inside the mitochondia to anaerobic reactions taking place in the cytoplasm outside mitochondia. Mills et al. suggest that early animals could have responded to low levels of oxygen inside the mitochondria indirectly with the help of sulfide, which increases as oxygen diminishes. Sulfide is removed by oxygen-dependent enzymes, which are blocked when oxygen is low, and Mills et al. show that these enzymes are conserved in all sponges, comb jellies and all other clades of animals.
On a more fundamental level, we do not know if the earliest animals were ever exposed to oxygen or needed to respond to changes in oxygen levels. However, a better knowledge of mitochondrial metabolism in sponges could provide more clues, since mitochondrial energy production is not solely driven by oxygen (Müller et al., 2012). Some modern animals, such as intracellular parasites and animals living in tidal zones or sulfide-rich environments, can switch from aerobic to anaerobic energy production in the mitochondria rather than in the cytoplasm, in order to deal with lack of oxygen. Looking ahead, it will be interesting to see if T. wilhelma or some other member of the sponge phylum (which contains over 8500 species; Van Soest et al., 2012) are indeed capable of anaerobic energy production in the mitochondria.
Dating early animal evolution using phylogenomic dataScientific Reports 7:3599.https://doi.org/10.1038/s41598-017-03791-w
Embracing uncertainty in reconstructing early animal evolutionCurrent Biology 27:R1081–R1088.https://doi.org/10.1016/j.cub.2017.08.054
Biochemistry and evolution of anaerobic energy metabolism in eukaryotesMicrobiology and Molecular Biology Reviews 76:444–495.https://doi.org/10.1128/MMBR.05024-11
BookPelagic Cnidarians and Ctenophores in Low Dissolved Oxygen Environments: A ReviewIn: Rabalais N. N, Turner R. E, editors. Coastal Hypoxia: Consequences for Living Resources and Ecosystems. Washington: American Geophysical Union. pp. 77–100.https://doi.org/10.1029/CE058p0077
Article and author information
- Version of Record published: February 6, 2018 (version 1)
- Version of Record updated: February 6, 2018 (version 2)
- Version of Record updated: April 7, 2020 (version 3)
© 2018, Rytkönen
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
- Page views
Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Oxygen was not the only factor that kick-started multicellular life.
- Developmental Biology
- Evolutionary Biology
Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors’ specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR/Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted toward photoreceptor or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species.