SET-9 and SET-26 are H3K4me3 readers and play critical roles in germline development and longevity
- Cornell University, United States
Abstract
C. elegans SET-9 and SET-26 are highly homologous paralogs that share redundant functions in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 with adjacent acetylation marks in vitroand in vivo. In the soma, SET-26 acts through DAF-16 to modulate longevity. In the germline, SET-9 and SET-26 restrict H3K4me3 domains around SET-9 and SET-26 binding sites, and regulate the expression of specific target genes, with critical consequence on germline development. SET-9 and SET-26 are highly conserved and our findings provide new insights into the critical role of these H3K4me3 readers in germline function and longevity.
Data availability
Sequencing data have been deposited in GEO under accession codes GSE108848 and GSE100623.
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SET-9 and SET-26 are H3K4me3 readers and play critical roles in germline development and longevityPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE108848).
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SET-9 and SET-26, the C. elegans homologs of human MLL5, are critical for germline development and longevityPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE100623).
Article and author information
Author details
Funding
National Institutes of Health (R01 grant AG024425)
- Siu Sylvia Lee
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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SET-9 and SET-26 are H3K4me3 readers and play critical roles in germline development and longevity
eLife 7:e34970.
https://doi.org/10.7554/eLife.34970
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