(a) Xenopus embryo: Simulated (Equation (1), Materials and methods) for a chromosome of length kb and a uniform distribution of p-oris (blue: kb/min, min, , kb) or a periodic distribution of p-oris (red: kb/min, min, , kb); (red squares) 3D simulations with the same parameter values as for periodic p-ori distribution; (black) experimental : raw data obtained from Goldar et al. (2009) were binned in groups of 4 data points; the mean value and standard error of the mean of each bin were represented. (b) S. cerevisiae: Simulated (Materials and methods) for the 16 chromosomes with the following parameter values: kb/min, , min-1, when considering only Confirmed origins (light blue), Confirmed and Likely origins (yellow) and Confirmed, Likely and Dubious origins (purple); the horizontal dashed lines mark the corresponding predictions for (Equation 5); (purple squares) 3D simulations with the same parameter values considering Confirmed, Likely and Dubious origins; (black) experimental from Goldar et al. (2009). (c) Eukaryotic organisms: as a function of ; (squares and bullets) simulations performed for regularly spaced origins (blue) and uniformly distributed origins (green) (Materials and methods) with two sets of parameter values: kb, kb/min, min and (dashed line) or (solid line); (black diamonds) experimental data points for Xenopus embryo, S. cerevisiae, S. cerevisae grown in Hydroxyurea (HU), S. pombe, D. melanogaster, human (see text and Table 1). The following figure supplement is available for Figure 2.