Radially-patterned cell behaviours during tube budding from an epithelium

Abstract
Data availability
Article and author information
Metrics

Abstract

The budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live-imaging and novel morphometric methods we show that in addition to apical constriction, radially-oriented directional intercalation of cells plays a major contribution to early stages of invagination of the salivary gland tube in the Drosophila embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell-wedging. Further from the pit cells interleave circumferentially, suggesting apically-driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges are biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding involves radially-patterned pools of apical myosin, medial as well as junctional, and radially-patterned 3D-cell behaviours, with a close mechanical interplay between invagination and intercalation.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for all figures.

Article and author information

Author details

Yara E Sanchez-Corrales

MRC-Laboratory of Molecular Biology, University of Cambridge, Cambridge, United Kingdom

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-1438-1994
Guy B Blanchard

Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom

For correspondence
gb288@cam.ac.uk

Competing interests
The authors declare that no competing interests exist.
Katja Röper

MRC -Laboratory of Molecular Biology, University of Cambridge, Cambridge, United Kingdom

For correspondence
kroeper@mrc-lmb.cam.ac.uk

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-3361-766X

Funding

Medical Research Council (U105178780)

Yara E Sanchez-Corrales
Guy B Blanchard
Katja Röper

Biotechnology and Biological Sciences Research Council (BB/J010278/1)

Guy B Blanchard

Wellcome (100329/Z/12/Z)

Guy B Blanchard

Isaac Newton Trust (15.23(k))

Guy B Blanchard

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.