Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells
- National Institutes of Health, United States
Abstract
The orientation of hair bundles on top of sensory hair cells (HCs) in neuromasts of the lateral line system allows fish to detect direction of water flow. Each neuromast shows hair bundles arranged in two opposing directions and each afferent neuron only innervate HCs of the same orientation. In previous paper, we show that this opposition is established by expression of Emx2 in half of the HCs, where it mediates hair bundle reversal (Jiang et al. 2017). Here, we show that Emx2 also regulates neuronal selection: afferent neurons innervate either Emx2-positive or negative HCs. In emx2 knockout and gain-of-function neuromasts, all HCs are unidirectional and the innervation patterns and physiological responses of the afferent neurons are dependent on the presence or absence of Emx2. Our results indicate that Emx2 mediates the directional selectivity of neuromasts by two distinct processes: regulating hair bundle orientation in HCs and selecting afferent neuronal targets.
Data availability
All data generated or analysed during this study are included in the manuscript. Source data files have been provided for Table1 and figure 2, 4, 5, 6, 7, and 8.
Article and author information
Author details
Funding
National Institute on Deafness and Other Communication Disorders (Intramural Research Program Grant 1ZIADC000085-01)
- Katie Kindt
National Institute on Deafness and Other Communication Disorders (Intramural Research Program Grant 1ZIADC000021)
- Doris K Wu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Metrics
-
- 2,413
- views
-
- 377
- downloads
-
- 35
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 35
- citations for umbrella DOI https://doi.org/10.7554/eLife.35796
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells
eLife 7:e35796.
https://doi.org/10.7554/eLife.35796
