TRPV4 is the temperature-sensitive ion channel of human sperm
Abstract
Ion channels control human sperm fertilizing ability by triggering hyperactivated motility, which is regulated by membrane potential, intracellular pH, and cytosolic calcium. Previous studies unraveled three essential ion channels that regulate these parameters: 1) the Ca2+ channel CatSper, 2) the K+ channel KSper, and 3) the H+ channel Hv1. However, the molecular identity of the sperm Na+ conductance that mediates initial membrane depolarization and, thus, triggers downstream signaling events is yet to be defined. Here, we functionally characterize DSper, the Depolarizing Channel of Sperm, as the temperature-activated channel TRPV4. It is functionally expressed at both mRNA and protein levels, while other temperature-sensitive TRPV channels are not functional in human sperm. DSper currents are activated by warm temperatures and mediate cation conductance, that shares a pharmacological profile reminiscent of TRPV4. Together, these results suggest that TRPV4 activation triggers initial membrane depolarization, facilitating both CatSper and Hv1 gating and, consequently, sperm hyperactivation.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for all figures.
Article and author information
Author details
Funding
National Institute of General Medical Sciences (R01GM111802)
- Polina V Lishko
Pew Charitable Trusts (28642)
- Polina V Lishko
Alfred P. Sloan Foundation (FR‐2015‐65398)
- Polina V Lishko
Deutscher Akademischer Austauschdienst
- Nadine Mundt
Packer Wentz Endowment Will
- Polina V Lishko
Rose Hill Innovator Fund
- Polina V Lishko
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Leon D Islas, Universidad Nacional Autónoma de México, Mexico
Ethics
Human subjects: The participation of healthy human sperm donor volunteers was approved by the Committee on Human Research at the University of California, Berkeley (protocol number 2013-06-5395). All donors provided informed consent.
Version history
- Received: February 11, 2018
- Accepted: June 30, 2018
- Accepted Manuscript published: July 2, 2018 (version 1)
- Accepted Manuscript updated: July 4, 2018 (version 2)
- Version of Record published: July 18, 2018 (version 3)
Copyright
© 2018, Mundt et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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