A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions
- University of Stuttgart, Germany
Abstract
Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCe that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions.
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All data generated or analysed during this study are included in the manuscript and supporting files.
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Funding
Deutsche Krebshilfe
- Angelika Hausser
Deutsche Forschungsgemeinschaft
- Angelika Hausser
Volkswagen Foundation
- Angelika Hausser
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Eisler et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions
eLife 7:e35907.
https://doi.org/10.7554/eLife.35907
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