(A) NC11-NC13 embryos expressing H2Av-GFP in interphase (A) or metaphase (A’). Scale bars represent 10 and 2 μm (inlay). (B) Quantitation of total nuclear H2Av-GFP fluorescent signal per nucleus in interphase and metaphase of NC11-NC13 embryos. Five nuclei were averaged per embryo, N = 3 embryos. NC11 vs NC12, p=0.0254; NC11 vs. NC13, p=0.0048; NC12 vs NC13, p=0.1214, NC11 metaphase vs. NC12 metaphase, p=0.0060; NC11 metaphase vs. NC13 metaphase, p=0.0001; NC12 metaphase vs. NC13 metaphase, p=0.0007. All p values were calculated using a two-way ANOVA followed by Tukey’s test. (C) Quantitation of FRAP experiments performed as described in Figure 6A on NC12 embryos injected with cycloheximide (red) or not (black). Nuclear levels continue to rise throughout a prolonged interphase. Five nuclei were averaged per embryo, N = 3 embryos. (D) Relative H2Av-GFP signal on metaphase chromosomes in NC13 wild-type and Jabba embryos. Scale bar represents 10 μm. (E) Quantitation of total signal within an equally sized ROI encompassing individual metaphase chromosomes. Five nuclei were averaged per embryo, N = 3 embryos. WT vs. Jabba, p=0.0011. All p values were calculated using an unpaired student t-test. All error bars represent SD.