(A) Western blots showing H3pT11 levels in WT (BY4741) cultures shifted from YPD to YP media containing 2% glucose, 3% glycerol, or 2% glucose with 3% glycerol (Glucose + Glycerol) for 1 hr. For ‘Glucose added’ cells, WT cultures were initially shifted from YPD to YP with 3% glycerol for 1 hr, were then directly supplied with 2% glucose, and were further incubated for 1 hr. (B) A bar plot showing the total number of H3pT11 peaks in YPglycerol (Glycerol-1 and 2) and YPD (Glucose-1 and 2) ChIP-seq experiments. This bar plot does not include peaks overlapping non-pol II genes or peaks on the mitochondrial chromosome. (C) The average profiles of H3pT11 around the transcription start site (TSS) and across the gene body for 139 genes whose H3pT11 levels are decreased in YPglycerol (Glycerol) compared to YPD (Glucose). tRNA and rRNA genes have been excluded. TES: transcription end site. (D) GO term analysis of the 139 genes, of which H3pT11 levels are decreased, from ChIP-sequencing data shown in (C).