Prognostication of chronic disorders of consciousness using brain functional networks and clinical characteristics
- The Chinese Academy of Sciences, China
- PLA Army General Hospital, China
- Guangzhou General Hospital of Guangzhou Military Command, China
Abstract
Disorders of consciousness are a heterogeneous mixture of different diseases or injuries. Although some indicators and models have been proposed for prognostication, any single method when used alone carries a high risk of false prediction. This study aimed to develop a multidomain prognostic model that combines resting state functional MRI with three clinical characteristics to predict one year outcomes at the single-subject level. The model discriminated between patients who would later recover consciousness and those who would not with an accuracy of around 88% on three datasets from two medical centers. It was also able to identify the prognostic importance of different predictors, including brain functions and clinical characteristics. To our knowledge, this is the first reported implementation of a multidomain prognostic model based on resting state functional MRI and clinical characteristics in chronic disorders of consciousness, which we suggest is accurate, robust, and interpretable.
Data availability
We have provided anonymised demographic and clinical characteristics of the DOC patients in Appendix 1. We have made the analysis pipeline, including fMRI preprocessing, feature calculation and extraction, regression and classification, and the results visualization publicly available. Also, we have uploaded the fMRI signals in each of region of interest for every DOC patient and healthy control involved in this study. Anyone is welcome to download them from GitHub (https://github.com/realmsong504/pDOC).
Article and author information
Author details
Funding
National Natural Science Foundation of China (81471380)
- Ming Song
National Natural Science Foundation of China (91432302,31620103905)
- Tianzi Jiang
The Science Frontier Program of the Chinese academy of Sciences (QYZDJ-SSW-SMC019)
- Tianzi Jiang
National Key R&D Program of China (2017YFA0105203)
- Tianzi Jiang
Beijing Municipal Science and Technology Commission (Z161100000216139)
- Tianzi Jiang
Beijing Municipal Science and Technology Commission (Z161100000216152)
- Ming Song
Beijing Municipal Science and Technology Commission (Z161100000516165)
- Ying Yang
The Guangdong Pearl River Talents Plan Innovative and Entrepreneurial Team (2016ZT06S220)
- Tianzi Jiang
Youth Innovation Promotion Association of the Chinese Academy of Sciences
- Ming Song
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Human subjects: The study was approved by the Ethics Committee of the PLA Army General Hospital (protocol no: 2011-097) and the Ethics Committee of the Guangzhou General Hospital of Guangzhou Military Command (protocol no: jz20091287). Informed consent to participate in the study was obtained from the legal surrogates of the patients and from the normal controls.
Copyright
© 2018, Song et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Prognostication of chronic disorders of consciousness using brain functional networks and clinical characteristics
eLife 7:e36173.
https://doi.org/10.7554/eLife.36173
Further reading
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Background:
It has been reported that loss of PCBP2 led to increased reactive oxygen species (ROS) production and accelerated cell aging. Knockdown of PCBP2 in HCT116 cells leads to significant downregulation of fibroblast growth factor 2 (FGF2). Here, we tried to elucidate the intrinsic factors and potential mechanisms of bone marrow mesenchymal stromal cells (BMSCs) aging from the interactions among PCBP2, ROS, and FGF2.
Methods:
Unlabeled quantitative proteomics were performed to show differentially expressed proteins in the replicative senescent human bone marrow mesenchymal stromal cells (RS-hBMSCs). ROS and FGF2 were detected in the loss-and-gain cell function experiments of PCBP2. The functional recovery experiments were performed to verify whether PCBP2 regulates cell function through ROS/FGF2-dependent ways.
Results:
PCBP2 expression was significantly lower in P10-hBMSCs. Knocking down the expression of PCBP2 inhibited the proliferation while accentuated the apoptosis and cell arrest of RS-hBMSCs. PCBP2 silence could increase the production of ROS. On the contrary, overexpression of PCBP2 increased the viability of both P3-hBMSCs and P10-hBMSCs significantly. Meanwhile, overexpression of PCBP2 led to significantly reduced expression of FGF2. Overexpression of FGF2 significantly offset the effect of PCBP2 overexpression in P10-hBMSCs, leading to decreased cell proliferation, increased apoptosis, and reduced G0/G1 phase ratio of the cells.
Conclusions:
This study initially elucidates that PCBP2 as an intrinsic aging factor regulates the replicative senescence of hBMSCs through the ROS-FGF2 signaling axis.
Funding:
This study was supported by the National Natural Science Foundation of China (82172474).
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