Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape
- Harvard TH Chan School of Public Health, United States
- Harvard Medical School, United States
- Swiss Federal Institute of Technology in Lausanne (EPFL), Switzerland
- Texas A&M University, United States
- Yale School of Medicine, United States
Abstract
In most well studied rod-shaped bacteria, peptidoglycan is primarily crosslinked by penicillin-binding proteins (PBPs). However, in mycobacteria, crosslinks formed by L,D-transpeptidases (LDTs) are highly abundant. To elucidate the role of these unusual crosslinks, we characterized Mycobacterium smegmatis cells lacking all LDTs. We find that crosslinks generate by LDTs are required for rod shape maintenance specifically at sites of aging cell wall, a byproduct of polar elongation. Asymmetric polar growth leads to a non-uniform distribution of these two types of crosslinks in a single cell. Consequently, in the absence of LDT-mediated crosslinks, PBP-catalyzed crosslinks become more important. Because of this, Mycobacterium tuberculosis (Mtb) is more rapidly killed using a combination of drugs capable of PBP- and LDT- inhibition. Thus, knowledge about the spatial and genetic relationship between drug targets can be exploited to more effectively treat this pathogen.
Data availability
Sequencing data were deposited into NCBI's Sequence Read Archive (SRA) under SRA study- SRP141343 https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP141343
Article and author information
Author details
Catherine Baranowski
Haig A Eskandarian
Thomas G Bernhardt
Funding
National Institutes of Health (U19 AI107774)
- Thomas R Ioerger
- Eric J Rubin
National Institutes of Health (R01 GM76710)
- Suzanne Walker
National Institutes of Health (R01AI083365)
- Thomas G Bernhardt
National Institutes of Health (F32GM123579)
- Michael A Welsh
Swiss National Science Foundation (205321_134786)
- Georg E Fantner
Innovative Medicines Initiative (115337)
- John McKinney
EU-FP7/Eurostars (E!8213)
- Georg E Fantner
European Molecular Biology Organization (750-2016)
- Haig A Eskandarian
National Science Foundation (DGE0946799)
- Karen J Kieser
National Institutes of Health (U19AI109764)
- Thomas G Bernhardt
Swiss National Science Foundation (205320_152675)
- Georg E Fantner
Burroughs Wellcome Fund (Career Award at the Scientific Interface)
- E Hesper Rego
American Heart Association (14POST18480014)
- Lok-To Sham
Simons Foundation (Fellow of the Life Sciences Research Foundation Award)
- Hoong C Lim
Swiss National Science Foundation (310030_156945)
- John McKinney
European Union (FP7/2007-2013/ERC Grant agreement No. 307338 (NaMic))
- Georg E Fantner
European Molecular Biology Organization (191-2014)
- Haig A Eskandarian
National Science Foundation (DGE1144152)
- Karen J Kieser
National Institutes of Health (F32AI104287)
- E Hesper Rego
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Bavesh D Kana, University of the Witwatersrand, South Africa
Version history
- Received: April 12, 2018
- Accepted: October 11, 2018
- Accepted Manuscript published: October 16, 2018 (version 1)
- Version of Record published: November 12, 2018 (version 2)
- Version of Record updated: September 5, 2019 (version 3)
Copyright
© 2018, Baranowski et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape
eLife 7:e37516.
https://doi.org/10.7554/eLife.37516
Further reading
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