E-cadherin binds to desmoglein to facilitate desmosome assembly
- Iowa State University, United States
- Center for Molecular Medicine Cologne, University of Cologne, Germany
- Emory University School of Medicine, United States
Figures
Figure 1
Ecad interacts with Dsg2 to form Ca2+-independent dimers.
(A) Schematic of specific interaction experiment. The AFM tip and substrate were functionalized with PEG linkers some of which were decorated with streptavidins. Biotinylated cadherin proteins were …
Figure 2
Lifetimes of the Ecad/Dsg2 dimer and the Dsc2/Dsc2 dimer are shorter than the lifetime of the Dsg2/Dsc2 complex.
Loading rates of the rupture events measured in Ca2+ at six different pulling velocities were grouped using K-means clustering method. Each clustered loading rate is shown by a different color, with …
Figure 3 with 1 supplement
Ecad and Dsg2 are both localized in nascent desmosomes.
(A) Analysis of cadherin localization within desmosomes. Structured illumination microscopy (SIM) is able to resolve the distance from plaque to plaque when desmosomes are stained with a C-terminal …
Figure 3—figure supplement 1
Relative Ecad levels remain unchanged over a calcium switch time-course.
Quantification of Ecad:DP ratio at cell borders. Entire cell border regions (in contrast to individual desmosomes as for Figure 3) were selected using the polygon selection tool in Fiji and the mean …
Figure 4
Ecad interacts with Dsg2 via Leu 175.
Homophilic binding probability of Ecad W2A-K14E double mutant (DM); heterophilic binding probability of DM and Dsg2; homophilic binding probability of Ecad L175D cis dimer mutant (CM); and the …
Figure 5 with 1 supplement
Ecad-L175 promotes desmosome assembly in cells.
(A) Immunofluorescence analysis for transfected WT or mutant Ecad (myc) and Dsg2, 6 hr after allowing de novo junction formation in EcadKO/PcadKD keratinocytes. Note decreased localization of Dsg2 …
Figure 5—figure supplement 1
Impaired junction formation in Ecad-K14E, Ecad-L175D, and Ecad-W2A/K14E (DM) mutants.
(A) Representation of the quantification criteria and junctional categories that were used in the analysis. Examples of cells transfected with Ecad mutant that do not (left panel) or do (right …
Figure 6
Proposed model for the role of Ecad in desmosome assembly.
Ecad (orange) interacts with Dsg2 (light blue) to form a low-lifetime cis heterodimer. Formation of Ecad/Dsg2 complex requires prior Ecad trans homodimerization. The Ecad/Dsg2 complex is …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.37629.010
