1. Chromosomes and Gene Expression
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An asymmetric centromeric nucleosome

  1. Yuichi Ichikawa
  2. Noriko Saitoh
  3. Paul D Kaufman  Is a corresponding author
  1. University of Massachusetts Medical School, United States
  2. The Cancer Institute of JFCR, Japan
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Cite this article as: eLife 2018;7:e37911 doi: 10.7554/eLife.37911

Abstract

Nucleosomes contain two copies of each core histone, held together by a naturally symmetric, homodimeric histone H3-H3 interface. This symmetry has complicated efforts to determine the regulatory potential of this architecture. Through molecular design and in vivo selection, we recently generated obligately heterodimeric H3s, providing a powerful tool for discovery of the degree to which nucleosome symmetry regulates chromosomal functions in living cells (Ichikawa et al., 2017). We now have extended this tool to the centromeric H3 isoform (Cse4/CENP-A) in budding yeast. These studies indicate that a single Cse4 N- or C-terminal extension per pair of Cse4 molecules is sufficient for kinetochore function, and validate previous experiments indicating that an octameric centromeric nucleosome is required for viability in this organism. These data also support the generality of the H3 asymmetric interface for probing general questions in chromatin biology.

Article and author information

Author details

  1. Yuichi Ichikawa

    Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6773-2455
  2. Noriko Saitoh

    Division of Cancer Biology, The Cancer Institute of JFCR, Tokyo, Japan
    Competing interests
    The authors declare that no competing interests exist.
  3. Paul D Kaufman

    Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States
    For correspondence
    paul.kaufman1@umassmed.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3089-313X

Funding

National Institutes of Health (R01GM100164)

  • Paul D Kaufman

National Institutes of Health (R35GM127035)

  • Paul D Kaufman

Japan Society for the Promotion of Science (JSPS KAKENHI 18H05531)

  • Noriko Saitoh

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Jerry L Workman, Stowers Institute for Medical Research, United States

Publication history

  1. Received: April 30, 2018
  2. Accepted: August 22, 2018
  3. Accepted Manuscript published: August 23, 2018 (version 1)
  4. Version of Record published: September 5, 2018 (version 2)
  5. Version of Record updated: September 11, 2018 (version 3)

Copyright

© 2018, Ichikawa et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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