H. polygyrus-infected Rag1-/- mice (RAG-/-) were given 0.4 µg recombinant IL-25 i.p. days 14–17 (late) post-infection with or without a complex of 5 μg rIL-4 and 25 μg anti-IL-4 (IL-4C) on days 13, 16 and 19 post-infection, according the schedule shown in (A). Mice were analyzed at 28 days post-infection for fecal egg burden (B) and intestinal adult worm burden (C). H. polygyrus-infected Rag1-/- mice were given IL-25 and IL-4C according to the same schedule, as well as 200 µg of anti-CD90.2/Th1.2 antibody or rat IgG2b control (days 12, 15, 18 and 21). The peritoneal lavage was analyzed at 28 days post-infection for CD45+lin- (CD3, CD5, CD8α, CD11c, CD19, DX5, F4/80, GR-1, TCRβ, CD11b), ICOS and ST2 staining by flow cytometry as shown (D) and the number of CD45+lin– (E), CD45+lin–ST2– (F) and CD45+lin–ST2+ (G) ILCs was determined. Mice were analysed at 28 days post-infection for fecal egg burden (H) and intestinal adult worm burden (I). Results shown are one representative of two experiments with n = 4 mice/group (D–G), or pooled data from two experiments with n ≥ 3 mice/group (B,C,H,I). Data were analysed by unpaired t test, where *=p≤0.05,**=p≤0.01,***=p≤0.001 and ns = not significant. Error bars represent Standard Error of the Mean.