Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit

There are several critical controls that need to be done in order for this paper to be a convincing contribution.

1) The genotypes in the figures suggest that Gal80^ts was not used for the anatomical experiment, but rather that FLP was activated developmentally for the anatomy. It is problematic to use the developmentally expressed FLP and loss of GFP signal to interpret the behavioral data, which is supposed to restrict FLP expression to the adult. Authors must show anatomical data for animals that had adult-specific FLP induction. Lack of phenotype for some of the Gal4s could be due to lack of adequate conversion.

This is indeed a valid point. We have now performed antibody staining with flies that were treated identical with flies used for memory experiments. We replaced the panels in the corresponding figures accordingly.

In addition, we conducted antibody staining with genotypes that did not show a LTM phenotype after CrebB deletion. CrebB::GFP removal from MB g neurons (5-HTR1B-Gal4) and PAM neurons (GMR58E02-Gal4) were confirmed and the results are shown in figure supplements (Figure 3—figure supplement 3 and Figure 4—figure supplement 1).

2) This raises a second point. The anatomical figures are of very low quality. Higher mag images and a counterstain for the areas where FLP is expressed are required to allow assessment of the efficacy of the adult-specific FLP outs.

We apologize for the quality of previous figures and have now performed a set of experiments to improve anatomical figures displayed at better resolution. The co-localization between CrebB and GFP (Figure 1 and Figure 1—figure supplement 1) and the FLP mediated CrebB::GFP knockout (Figure 2 and Figure 3) can be better assessed in the new images.

3) The authors also need to do temperature controls. They should show how experimental flies behave (STM and LTM) when kept at the low temperature through development and adulthood. This is important for the conclusion that the manipulation of CrebB was restricted to the adult fly and that this changed LTM.

It is indeed true that testing memory performance of flies with non-induced FLP expression is an important control. We have now conducted non-induced FLP expression control experiments, in which flies were kept continuously at 18°C, for all the used genotypes. The data is displayed in figure supplements.