(A) CryoEM maps of the PIC py48S-eIF5N shown in three orientations. Regions of the map are colored by component to show the 40S subunit (yellow), eIF1A (blue), eIF5-NTD (cyan), Met-tRNAiMet (green), mRNA (magenta), eIF2α (violet), eIF2γ (orange), eIF2β (red), eIF3 (different shades of pink). The 40S head is shown in a darker yellow compared to the body. The density for 40S, eIF1A, mRNA, tRNA, eIF2 subunits and eIF5 is taken from Map C1, whereas density for eIF3 PCI domains is taken from Map A, and for eIF3-bgi subcomplex from Map B. The same colors are used in all the figures. (B) Atomic model for the PIC in the same colors and in the same three orientations. See also Figure 1—figure supplements 1, 2, 3, 4, 5 and 6.