(A, C, H, J) y- (A, H) and x-velocity (C, J) kymographs from three independent experiments with 15 min binning of three 5 min PIV data of movements of MDCK E-cadherin:DsRed cells treated with 50 μM blebbistatin (+Blebb) (A, C) or of MDCK T151 cells (H, J) and sheared (dashed black line) over 20 hr. (A–D) Blebbistatin was added 15 min before shear (filled arrowhead) and washout one hour later (empty arrowhead). (B, D, I, K) y- (B and I) and x-direction (D and K) cell movements at positions 8, 50, 100, 150, 200, and 242 μm from the shear-plane for +Blebb MDCK cells (B, D) and T151 cells (J, K), with insets providing greater spatial resolution of passive movement in the deformation zone (D and K, insets). (E, L) Measured resistive force of the epithelium over 30 min in response to 100 μm shear displacement for +Blebb MDCK cells (E) and T151 cells (L) (dashed line, time of shear; solid blue bar, 63.2% decay time constant τ; Figure 2—figure supplement 2). (F, M) Values of the mechanical model elements presented in Figure 2B (+Shear) were increased (blue) or decreased (red) to capture the mechanics and movement of the epithelium. (G, N) (Experiment) Unbinned (5 min) y-velocity kymographs for +Blebb MDCK cells (G) and T151 cells (N) were averaged in the y-direction to obtain the overall y-direction cell velocities at each time point with shear (dashed black line). The range (gray bars) and mean (black crosses) of three independent experiments at each time point (E, G, L, N) are shown. (Simulation) Normalized output of the MATLAB Simulink/Simscape simulation for the mechanical model (Figure 2—figure supplement 1). Kymograph assembly, color maps, data binning, and numerical integration over time were as in Figure 1.