CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors

  1. Manuela D'Alessandro
  2. Magali Richard
  3. Christian Stigloher
  4. Vincent Gache
  5. Thomas Boulin
  6. Janet E Richmond
  7. JeanLouis Bessereau  Is a corresponding author
  1. University of Lyon - INSERM - CNRS, France
  2. University of Illinois at Chicago, United States

Abstract

The assembly of neurotransmitter receptors in the endoplasmic reticulum limits the number of receptors delivered to the plasma membrane, ultimately controlling neurotransmitter sensitivity and synaptic transfer function. In a forward genetic screen conducted in the nematode C. elegans, we identified crld-1 as a gene required for the synaptic expression of ionotropic acetylcholine receptors (AChR). We demonstrated that the CRLD-1A isoform is a membrane-associated ER-resident protein disulfide isomerase (PDI). It physically interacts with AChRs and promotes the assembly of AChR subunits in the ER. Mutations of Creld1, the human ortholog of crld-1a, are responsible for developmental cardiac defects. We showed that Creld1 knockdown in mouse muscle cells decreased surface expression of AChRs and that expression of mouse Creld1 in C. elegans rescued crld-1a mutant phenotypes. Altogether these results identify a novel and evolutionarily-conserved maturational enhancer of AChR biogenesis, which controls the abundance of functional receptors at the cell surface.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Manuela D'Alessandro

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Magali Richard

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Christian Stigloher

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6941-2669
  4. Vincent Gache

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Thomas Boulin

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    Competing interests
    The authors declare that no competing interests exist.
  6. Janet E Richmond

    Department of Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  7. JeanLouis Bessereau

    Institut NeuroMyoGene, University of Lyon - INSERM - CNRS, Lyon, France
    For correspondence
    jean-louis.bessereau@univ-lyon1.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3088-7621

Funding

Association Française contre les Myopathies (Post-doctoral Fellowship 16451)

  • Manuela D'Alessandro

European Molecular Biology Organization (Long term Post-doctoral fellowship)

  • Christian Stigloher

Institut National de la Santé et de la Recherche Médicale (Junior Grant)

  • Thomas Boulin

Association Française contre les Myopathies (Myoneuralp)

  • JeanLouis Bessereau

Deutscher Akademischer Austauschdienst (Postdoctoral Program of the German Academic Exchange Service)

  • Christian Stigloher

Fédération pour la Recherche sur le Cerveau (Operation Espoir en tête 2013)

  • JeanLouis Bessereau

Fondation ARC pour la Recherche sur le Cancer (4th year PhD program 2011)

  • Magali Richard

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Oliver Hobert, Howard Hughes Medical Institute, Columbia University, United States

Version history

  1. Received: July 12, 2018
  2. Accepted: November 5, 2018
  3. Accepted Manuscript published: November 8, 2018 (version 1)
  4. Version of Record published: November 20, 2018 (version 2)

Copyright

© 2018, D'Alessandro et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Manuela D'Alessandro
  2. Magali Richard
  3. Christian Stigloher
  4. Vincent Gache
  5. Thomas Boulin
  6. Janet E Richmond
  7. JeanLouis Bessereau
(2018)
CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors
eLife 7:e39649.
https://doi.org/10.7554/eLife.39649

Share this article

https://doi.org/10.7554/eLife.39649

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