Dopamine neurons projecting to medial shell of the nucleus accumbens drive heroin reinforcement

  1. Julie Corre
  2. Ruud van Zessen
  3. Michaël Loureiro
  4. Tommaso Patriarchi
  5. Lin Tian
  6. Vincent Pascoli
  7. Christian Lüscher  Is a corresponding author
  1. University of Geneva, Switzerland
  2. University of California Davis, United States
  3. University of Geneva Hospital, Switzerland
6 figures, 1 table and 2 additional files

Figures

Heroin self-administration.

(a), Schematic of behavioral setup for self-administration experiments in (d-g). (b), Day-to-day schedule of experiment for (d–g). (c), Detail of the sequence of events following a press on the …

https://doi.org/10.7554/eLife.39945.002
Heroin increases DA levels in the NAc via enhanced VTA DA neuron activity.

(a) Fluorescence in response to five neuromodulators in HEK293 cells expressing dLight1 (DYN: dynorphin; GLU: glutamate; 5HT: serotonin; NE: norepinephrine). Data are presented as median with 25/75 …

https://doi.org/10.7554/eLife.39945.003
Heroin activates NAc projecting DA neurons in the medial VTA.

(a), Schematic of experiment for (b-e); (b), TH (left, red), cFos (middle, green) staining of VTA DA neurons and co-localization of TH- and cFos-expressing neurons (right) after one day of either …

https://doi.org/10.7554/eLife.39945.004
Chemogenetic inhibition of VTA DA neurons suppresses heroin self-administration.

(a), Schematic of the experiment for (b-e); (b), Left, VTA of DTA-Cre+ mice were bilaterally injected with the inhibitory DREADD hM4D. Right, coronal confocal images of infected VTA. (c), Raster …

https://doi.org/10.7554/eLife.39945.005
Heroin occludes optogenetic self-stimulation of VTA DA neurons.

(a), Schematic of the experiment for (b-f); (b), Left, VTA of DAT-Cre+ mice was bilaterally injected with a floxed version of the excitatory opsin ChR2. Right, coronal confocal images of infected …

https://doi.org/10.7554/eLife.39945.006
Heroin occludes reinforcing effects of self-inhibition of VTA GABA neurons.

(a), Schematic of the experiment for b-f; (b), Left, VTA of GAD-Cre+ mice was bilaterally injected with a floxed version of the inhibitory opsin Arch3.0. Right, coronal confocal images of infected …

https://doi.org/10.7554/eLife.39945.007

Tables

Key resources table
Reagent type
(species) or
resource
DesignationSource or
reference
IdentifiersAdditional
information
Genetic reagent (M. musculus)DATIREScreThe Jackson Laboratory (www.jax.org)MGI:3689434
Genetic reagent (M. musculus)Gad2tm2(cre)ZjhThe Jackson Laboratory (http://www.jax.org)MGI:4418713
Cell line (homo sapiens, human)HEK293TATCCCat# CRL-1573
Recombinant DNA reagentAAV9-CAG-dLight1.1Dr. Lin Tian, University of California DavisPatriarchi et al., 2018
Recombinant DNA reagentAAVDJ-EF1a-DIO-GCaMP6mStanford Vector CoreCat# GVVC-AAV-94
Recombinant DNA reagentAAV8-hSyn-DIO-ChrimsonR-tdToUNC Vector Core
Recombinant DNA reagentAAV5-hSyn-DIO-HM4D(Gi)-mCherryUNC Vector Core
Recombinant DNA reagentAAV5-EF1a-DIO-ChR2(H134R)-eYFPUNC Vector Core
Recombinant DNA reagentAAV5-EF1a-DIO-eArch3.0-eYFPUNC Vector Core
Peptide, recombinant proteinCTB (Alexa FluorTM 555 Conjugate)Invitrogen/Thermo FisherCat# C34776
Peptide, recombinant proteinCTB (Alexa FluorTM 488 Conjugate)Invitrogen/Thermo FisherCat# C34775
Peptide, recombinant proteinCTB (Alexa FluorTM 647 Conjugate)Invitrogen/Thermo FisherCat# C34778
Chemical compound, drugDiacetylmorphine (heroin)DiaMo Narcotics GmbHDIAPHIN
Chemical compound, drugCitalopramCayman ChemicalCat# 14572
Chemical compound, drugReboxetineTocrisCat# 1982
Chemical compound, drugCocaineUniversity Hospital of Geneva
AntibodyAnti-cFosSanta Cruz BiotechnologyRRID: AB_2106783(dilution 1:5000)
AntibodyAnti-Tyrosine HydroxylaseSigma-AltrichCat# T2928(dilution 1:500)
AntibodyAnti- GFPInvitrogen/Thermo FisherCat# 11122(dilution 1:500)
Software, algorithmPrism 7.02Graphpad
Software, algorithmMATLAB R2017aMathworks
Software, algorithmSynapseTucker-Davis Technologies

Additional files

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