(A) Schematic overview of the assumed sexual process between two parental strains of Z. tritici (Alexopoulos et al., 1996; Kema et al., 2018) with one supernumerary chromosome shared and therefore …
(A) Synteny blot for IPO94269 unitigs on IPO323 chromosomes. (B) Summary table of syntenic regions of IPO323 and IPO94269.
(A) Relative frequencies of mitochondrial genotypes in random and randomized ascospores in experiments A, B and C. The mitochondrial transmission varied significantly between the three experiments. …
(A) Detailed presence/absence frequencies for all unpaired supernumerary chromosomes according to the mitochondrial genotype present in all ascospores. Data for the three experiments A, B and C are …
Analysis of segregation of paired supernumerary chromosomes in 24 complete tetrads. The transmission of chromosomes 14, 15, 16, 17, 19, and 21 with homologs in both parental strains IPO94269 and …
Examples of gel electrophoresis of PCR products for (A) core chromosome 13 marker on mating type, (B) core chromosome 4 marker 11O21, (C) core chromosome 4 marker 04L20, (D) core chromosome three marker caa-0002, core chromosome five marker ggc-001, core chromosome seven marker ac-001, (E) segregating marker of the mitochondrial genotype, (F) segregating marker of the supernumerary chromosome 14, (G) segregating marker of the supernumerary chromosome 15, (H) segregating marker of the supernumerary chromosome 16, (I) segregating marker of the supernumerary chromosome 17, (J) segregating marker of the supernumerary chromosome 19, (K) segregating marker of the supernumerary chromosome 19, (L) subtelomeric marker of the supernumerary chromosome 19, (M) subtelomeric marker of the supernumerary chromosome 19, (N) segregating marker of the supernumerary chromosome 21, (O) subtelomeric marker of the supernumerary chromosome 18, (P) centromeric marker of the supernumerary chromosome 18, (Q) subtelomeric marker of the supernumerary chromosome 18, (R) subtelomeric marker of the supernumerary chromosome 20, (S) centromeric marker of the supernumerary chromosome 20, (T) subtelomeric marker of the supernumerary chromosome 20.
Coverage heatmap for eight ascospores (T0325-T0332) of ascus A03-4 (A–C) and eight ascospores (T0801-T0808) of ascus A08-1 (D–F). (a) Supernumerary chromosome complement in cross resulting in ascus …
The distribution is consistent with the origin of each tetrad in one meiotic event and Mendelian segregation for core and paired supernumerary chromosomes. (A) Distribution of IPO94269 specific SNP …
Analysis of segregation of unpaired supernumerary chromosomes in 24 complete tetrads according to the mitochondrial genotype. The transmission of chromosomes unique to one of the parental strains …
Schematic illustration depicting two possible mechanisms for the observed meiotic chromosome drive of female derived unpaired supernumerary chromosomes. Light blue/orange: paired supernumerary …
A) Whole genome view of all eight ascopsores of ascus A03-4. Stretches of IPO94269 haplotype alternate with stretches of IPO323 haplotype. Crossover events are discernible between all eight …
Ascospores (random*/all) | Verified tetrads (mtIPO323/mtIPO94269) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
# | Parental strain 1 | Parental strain 2 | Unpaired chr from IPO94269 | Unpaired chr from IPO323 | Condition | Exp A | Exp B | Exp C | Exp A | Exp B | Exp C |
1 | IPO323 | IPO94269 | - | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 12/96 | 51/88 | - | 2/2 | 0 |
2 | IPO323 ∆chr14 | IPO94269 | chr14 | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 8/64 | 38/72 | - | 3/0 | 1/0 |
3 | IPO323 ∆chr21 | IPO94269 | chr21 | chr18, chr20 | Co-inoculation 107 cells/mL | 89/89 | 4/32 | 52/78 | - | 0 | 0 |
4 | IPO323 ∆chr16 | IPO94269 | chr16 | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 6/48 | 38/115 | - | 1/0 | 0/1 |
5 | IPO323 ∆chr17 | IPO94269 | chr17 | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 2/16 | 15/59 | - | 0 | 2/0 |
5 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 9/72 | 38/77 | - | 3/1 | 0/1 |
7 | IPO323 ∆chr20 | IPO94269 | - | chr18 | Co-inoculation 107 cells/mL | 96/96 | 4/32 | 19/31 | - | 2/0 | 0 |
8 | IPO323 ∆chr18 | IPO94269 | - | chr20 | Co-inoculation 107 cells/mL | 96/96 | 4/32 | 30/67 | - | 1/0 | 2/0 |
9 | IPO323 ∆chr15 | IPO94269 | chr15 | chr18, chr20 | Co-inoculation 107 cells/mL | 96/96 | 6/48 | 14/54 | - | 1/0 | 0/1 |
10 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO323 106 cells/mL | - | - | 25/42 | - | - | 0 |
11 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO323 105 cells/mL | - | - | 38/43 | - | - | 0 |
12 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO323 104 cells/mL | - | - | 2/16 | - | - | 0 |
13 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO94269 +6dpi | - | - | 40/46 | - | - | 0 |
14 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO94269 +12dpi | - | - | 11/11 | - | - | 0 |
15 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO94269 106 cells/mL | - | - | 60/84 | - | - | 0 |
16 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO94269 105 cells/mL | - | - | 48/80 | - | - | 0 |
17 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO94269 104 cells/mL | - | - | 28/28 | - | - | 0 |
18 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO323 +6pdi | - | - | 5/29 | - | - | 0 |
19 | IPO323 ∆chr19 | IPO94269 | chr19 | chr18, chr20 | IPO323 +12dpi | - | - | 51/71 | - | - | 1/0 |
∑ | 761/761 | 55/440 | 603/1091 | - | 13/3 | 5/3 |
*Includes random and randomized ascospores. Randomized ascospores were generated by randomly selecting one ascospore per tetrad.
Marker | Localization (IPO323) | Localization (IPO94269) | Primer1 | Primer 2 | Product size in IPO323 [bp] | Product size in IPO94269 [bp] |
---|---|---|---|---|---|---|
mat1-1/mat1-2 | chr13 621930–622924 | Unitig11 627136–627792 | MAT1-1F, MAT1-1R | MAT1-2F, MAT1-2R | 340 | 660 |
11O21 | chr4 642791–642996 | Unitig03 757794–757990 | 11O21F | 11O21R | 205 | 199 |
04L20 | chr4 2276298–2276497 | Unitig03 2427163–2427370 | 04L20F | 04L20R | 192 | 199 |
caa-0002 | chr3 2927294–2927704 | Unitig02 566646–567041 | 2996 | 2997 | 412 | 396 |
ggc-001 | chr5 1190388–1190640 | Unitig04 1143568–1143802 | 2998 | 2999 | 254 | 234 |
ac-0001 | chr7 266661–266847 | Unitiq05 372979–373151 | 3000 | 3001 | 187 | 173 |
All primers used in this study.
Summary of all PCR marker results for experiment A.
Summary of all PCR marker results for experiment B.
Summary of all PCR marker results for experiment C.
Summary of statistical tests performed in this study.
Frequency of transmission of paired chromosomes to progeny ascospores.