Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway

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Abstract

High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.

Data availability

Coordinates and structure factors have been deposited in the PDB with the accession codes: 6DSU, 6DSV, 6DSW, 6DSX, and 6DSY.

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Nicholas Chim

Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, United States

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-2274-5305
Lynnette N Jackson

Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, United States

Competing interests
The authors declare that no competing interests exist.
Anh M Trinh

Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, United States

Competing interests
The authors declare that no competing interests exist.
John C Chaput

Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, United States

For correspondence
jchaput@uci.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-1393-135X

Funding

Defense Advanced Research Projects Agency (N66001-16-2-4061)

John C Chaput

National Science Foundation (1607111)

John C Chaput

National Institutes of Health (R25GM055246)

Lynnette N Jackson

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.