Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway
- University of California, Irvine, United States
Abstract
High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.
Data availability
Coordinates and structure factors have been deposited in the PDB with the accession codes: 6DSU, 6DSV, 6DSW, 6DSX, and 6DSY.
Article and author information
Author details
Funding
Defense Advanced Research Projects Agency (N66001-16-2-4061)
- John C Chaput
National Science Foundation (1607111)
- John C Chaput
National Institutes of Health (R25GM055246)
- Lynnette N Jackson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- John Kuriyan, University of California, Berkeley, United States
Version history
- Received: July 25, 2018
- Accepted: October 18, 2018
- Accepted Manuscript published: October 19, 2018 (version 1)
- Version of Record published: November 12, 2018 (version 2)
Copyright
© 2018, Chim et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway
eLife 7:e40444.
https://doi.org/10.7554/eLife.40444
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