Pervasive transcription fine-tunes replication origin activity
- Université Paris-Diderot, France
Abstract
RNA polymerase (RNAPII) transcription occurs pervasively, raising the important question of its functional impact on other DNA-associated processes, including replication. In budding yeast, replication originates from Autonomously Replicating Sequences (ARSs), generally located in intergenic regions. The influence of transcription on ARSs function has been studied for decades, but these earlier studies have neglected the role of non-annotated transcription. We studied the relationships between pervasive transcription and replication origin activity using high-resolution transcription maps. We show that ARSs alter the pervasive transcription landscape by pausing and terminating neighboring RNAPII transcription, thus limiting the occurrence of pervasive transcription within origins. We propose that quasi-symmetrical binding of the ORC complex to ARS borders and/or pre-RC formation are responsible for pausing and termination. We show that low, physiological levels of pervasive transcription impact the function of replication origins. Overall, our results have important implications for understanding the impact of genomic location on origin function.
Data availability
All data analyzed in this manuscript have been previously published and appropriate GEO accession codes and references have been provided.
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Genome-wide mapping of yeast RNA polymerase II termination.NCBI Gene Expression Omnibus, GSE56435.
Article and author information
Author details
Funding
Centre National de la Recherche Scientifique
- Domenico Libri
Fondation pour la Recherche Médicale (F.R.M. programme équipes 2013)
- Domenico Libri
Agence Nationale de la Recherche (Grant ANR-16-CE12-0022-01)
- Domenico Libri
Labex Who Am I? (ANR-11-LABX-0071)
- Domenico Libri
French Ministry of Research (Fellowship)
- Tito Candelli
Ligue Nationale Contre le Cancer (GB/MA/CD/IQ - 12031)
- Julien Gros
Labex Who Am I? (ANR-11-IDEX-0005-02)
- Domenico Libri
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Bruce Stillman, Cold Spring Harbor Laboratory, United States
Version history
- Received: August 6, 2018
- Accepted: December 17, 2018
- Accepted Manuscript published: December 17, 2018 (version 1)
- Version of Record published: January 2, 2019 (version 2)
Copyright
© 2018, Candelli et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Pervasive transcription fine-tunes replication origin activity
eLife 7:e40802.
https://doi.org/10.7554/eLife.40802
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